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Invitrogen
SCAP Polyclonal Antibody
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FIGURE: 1 / 4
SCAP Antibody (PA5-115869) in ICC/IF
Immunocytochemistry analysis of SCAP in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115869) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-115869
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SCAP(Accession Q12770), corresponding to amino acid residues K646-G696.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total SCAP.
Target Information
The protein encoded by this gene interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements. This protein contains a bromodomain and is thought to be a nuclear receptor coactivator. Three alternatively spliced transcript variants that encode distinct isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: KIAA0199; Sreb cleavage-activating protein; SREBP cleavage activating protein; SREBP cleavage-activating protein; unnamed protein product
Gene Aliases: 9530044G19; mKIAA0199
UniProt ID: (Human) Q12770, (Mouse) Q6GQT6, (Rat) A2RRU4
Entrez Gene ID: (Human) 22937, (Mouse) 235623, (Rat) 301024
regulation of transcription from RNA polymerase II promoter
cholesterol metabolic process
SREBP signaling pathway
response to vitamin B3
regulation of cholesterol biosynthetic process
negative regulation of cholesterol biosynthetic process
positive regulation of cholesterol biosynthetic process
cargo loading into COPII-coated vesicle
response to hypoxia
lipid metabolic process
immune response
regulation of fatty acid metabolic process
protein catabolic process
response to insulin
regulation of fatty acid biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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