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Invitrogen
SCF Polyclonal Antibody
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FIGURE: 1 / 1
SCF Antibody (PA5-19793) in IHC (P)
Immunohistochemical (formalin-fixed, paraffin-embedded) staining of Human Normal Colon tissue using Product # PA5-19793, anti-SCF antibody. Primary antibody was used at a concentration of 5 µg/mL and exposed for 15 mins at room temp. The sample was pretreated using heat mediated antigen retrieval with Sodium Citrate Buffer (pH6/20mins). The detection method was a HRP conjugated polymer, DAB chromogen and th... View More
Product Details
PA5-19793
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SCF.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
For Western Blot, this antibody has non-specific bands at 120 kDa and 50 kDa.
Heat mediated antigen retrieval recommended prior to tissue staining.
This antibody is predicted to react with rat, sheep, goat, horse, cow, cat, dog, pig and rhesus monkey based on sequence homology.
Target Information
Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-Kit ligand; DKFZp686F2250; familial progressive hyperpigmentation 2; growth factor; H-SCF; Kit ligand; kit-ligand; M-SCF; Mast cell growth factor; MGF; polypeptide growth factor; SCF; SCF220; SCF248; steel factor; Stem cell factor; unnamed protein product
Gene Aliases: DCUA; DFNA69; FPH2; FPHH; Kitl; KITLG; KL-1; MGF; SCF; SF; SHEP7; SLF; WS2F
UniProt ID: (Human) P21583
Entrez Gene ID: (Human) 4254
ovarian follicle development
neural crest cell migration
hematopoietic progenitor cell differentiation
myeloid leukocyte differentiation
positive regulation of leukocyte migration
positive regulation of myeloid leukocyte differentiation
developmental process involved in reproduction
apoptotic process
cell adhesion
Ras protein signal transduction
cell proliferation
positive regulation of cell proliferation
male gonad development
mast cell apoptotic process
negative regulation of mast cell apoptotic process
embryonic hemopoiesis
ectopic germ cell programmed cell death
T cell proliferation
positive regulation of T cell proliferation
negative regulation of apoptotic process
positive regulation of melanocyte differentiation
positive regulation of Ras protein signal transduction
animal organ development
mast cell proliferation
positive regulation of mast cell proliferation
extrinsic apoptotic signaling pathway in absence of ligand
melanocyte migration
mast cell migration
positive regulation of hematopoietic progenitor cell differentiation
positive regulation of hematopoietic stem cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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