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Invitrogen
SCRIB Polyclonal Antibody
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FIGURE: 1 / 5
SCRIB Antibody (PA5-116752) in ICC/IF
Immunofluorescence analysis of SCRIB was performed using 70% confluent log phase HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SCRIB Polyclonal Antibody (Product # PA5-116752) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:... View More
Product Details
PA5-116752
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1100-1400 of human SCRIB (NP_8743653)
Conjugate
Form
Liquid
Concentration
1.19 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: A-549, 293T
Immunogen sequence: ELCIQKAPGE RLGISIRGGA RGHAGNPRDP TDEGIFISKV SPTGAAGRDG RLRVGLRLLE VNQQSLLGLT HGEAVQLLRS VGDTLTVLVC DGFEASTDAA LEVSPGVIAN PFAAGIGHRN SLESISSIDR ELSPEGPGKE KELPGQTLHW GPEATEAAGR GLQPLKLDYR ALAAVPSAGS VQRVPSGAAG GKMAESPCSP SGQQPPSPPS PDELPANVKQ AYRAFAAVPT SHPPEDAPAQ PPTPGPAASP EQLSFRERQK YFELEVRVPQ AEGPPKRVSL VGADDLRKMQ EEEARKLQQK R
Target Information
SCRIB is a cytoplasmic multimodular scaffold protein targeted to epithelial adherens junctions and neuronal presynaptic compartments. SCRIB and its orthologs in vertebrates and invertebrates participate in cell polarization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hScrib; Protein LAP4; Protein scribble homolog; S Vartul; Scribble; scribbled homolog
Gene Aliases: CRIB1; KIAA0147; LAP4; SCRB1; SCRIB; SCRIB1; VARTUL
UniProt ID: (Human) Q14160
Entrez Gene ID: (Human) 23513
neural tube closure
positive regulation of receptor recycling
asymmetric protein localization
cell proliferation
viral process
synaptic vesicle targeting
single organismal cell-cell adhesion
cell migration
cochlear nucleus development
establishment of apical/basal cell polarity
wound healing
positive regulation of apoptotic process
astrocyte cell migration
negative regulation of mitotic cell cycle
synaptic vesicle endocytosis
positive chemotaxis
auditory receptor cell stereocilium organization
apoptotic process involved in morphogenesis
mammary gland duct morphogenesis
protein localization to adherens junction
activation of GTPase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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