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Rockland
SCUBE3 Polyclonal Antibody
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FIGURE: 1 / 1
SCUBE3 Antibody (600-401-EF1) in IHC (P)
Immunohistochemistry of SCUBE3 antibody. Tissue: Blood vessel tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: SCUBE3 antibody at 10 µg/mL for 1 hr at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: SCUBE3 is secreted into the extracellular space. Staining: SCUBE3 as precipitated purple signal with... View More
Product Details
600-401-EF1
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-SCUBE3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid peptide near the internal region of human SCUBE3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
SCUBE3 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and is highly expressed in primary osteoblasts and bones, and to a lesser extent in heart. Other studies have shown that overexpression of SCUBE3 in mice induced cardiac hypertrophy, suggesting that it may also play a role in the regulation of cardiac growth. SCUBE3 has been shown to be an endogenous TGF-Beta receptor ligand and is thought to promote lung cancer cell mobility and invasiveness. In lung cancer cells, the secreted SCUBE3 protein was cleaved by MMP2 and MMP9, allowing the activation of the TGF-Beta receptor, the increase of Smad2/3 transcriptional activity and the upregulation of expression of proteins such as TGF-Beta1, VEGF, Snail, and Slug.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CUB domain and EGF-like repeat containing 3; signal peptide; Signal peptide, CUB and EGF-like domain-containing protein 3
Gene Aliases: CEGF3; SCUBE3
UniProt ID: (Human) Q8IX30
Entrez Gene ID: (Human) 222663
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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