|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide made to an N-terminal region of the human SDHB protein (within residues 1-150).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 30% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:7000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The target sequence has 92% sequence homology with rat, bovine and porcine, 85% sequence homology with xenopus and 84% sequence homology with zebrafish.
Part of the succinate dehydrogenase complex, SDHB is a mitochondrial protein that interfaces with the catalysis of succinate dehydrogenase. In many gastrointestinal tumors, SDHB is irregularly expressed, and leads to mutational activation of KIT. This may be as a result of elevated oxidative stress caused by mitochondrial dysfunction. In Dutch populations, there is a higher prevalence of mutations in the SDHB gene, and a higher overall level of hereditary paraganglioma and pheochromocytoma syndromes than in other Europeans. Similar trends in mutations in this gene seem to be related to atherosclerosis in American populations. Overall, the SDHB protein effects multiple cellular metabolism processes and it is not surprisingly linked to metabolism-related cellular dysfunctions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.