Invitrogen

SETMAR Polyclonal Antibody

View all (7) SETMAR antibodies

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Datasheet

Protocols

Questions & Answers

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SETMAR Antibody in Western Blot (WB) — Western blot analysis was performed on Nuclear extracts (30 µg lysate) of SH-SY5Y (Lane 1), HEL 92.1.7 (Lane 2), NTERA-2 (Lane 3) and U-2 OS (Lane 4). The blots were probed with Anti-SETMAR Rabbit Polyclonal Antibody (Product # 720343, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Two isoforms of 78 kDa, 41 kDa (cell lines) and 62 kDa (mouse muscle) corresponding to SETMAR was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Product Details

720343

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Protein corresponding to human SETMAR [aa15-aa303] View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 30% glycerol, 0.1% BSA
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2663480

Product Specific Information

This antibody is predicted to react with Monkey, Bovine, Sheep

Target Information

SETMAR (SET domain and mariner transposase fusion gene) is a histone methyltransferase that methylates Lys-4 and Lys-36 of histone H3, creating specific tags for epigenetic transcriptional activation. It specifically mediates dimethylation of H3 Lys-36. SETMAR has sequence-specific DNA-binding activity, and recognizes the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 transposable element. SETMAT has DNA nicking activity, and in vivo end-joining activity, and may mediate genomic integration of foreign DNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: epidermal tumor expressed transcript 2; Histone-lysine N-methyltransferase; Histone-lysine N-methyltransferase SETMAR; Metnase; SET domain and mariner transposase fusion gene-containing protein; SET domain and mariner transposase fusion gene-containing protein homolog; SET domain and mariner transposase fusion protein; SET domain without mariner transposase fusion protein; Transposon Hsmar1 transposase

Gene Aliases: 5830404F24Rik; Etet2; Mar1; METNASE; SETMAR

UniProt ID: (Human) Q53H47, (Mouse) Q80UJ9

Entrez Gene ID: (Human) 6419, (Mouse) 74729

Molecular Function: histone modifying enzyme viral or transposable element protein