Invitrogen

SFMBT1 Polyclonal Antibody

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SFMBT1 Antibody in Western Blot (WB) — Western Blot using SFMBT1 Polyclonal Antibody (Product # PA5-22393). Sample (30 µg of whole cell lysate). Lane A: IMR32. 7.5% SDS PAGE. SFMBT1 Polyclonal Antibody (Product # PA5-22393) diluted at 1:3,000.

SFMBT1 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Distribution of SFMBT1 in colorectal cancer (CRC) tumor tissues and cells. A Western blot analysis of SFMBT1 protein levels in normal and tumor tissues. ( n = 6) ( B ) Expression of SFMBT1 in normal and CRC tumor tissues were examined by immunohistochemistry. C Expression of SFMBT1 in CRC tissue microarrays. D , E TCGA data shows the expression of SFMBT1 in tumor tissues and normal tissues or in different cancer stages. F , G qRT-PCR ( F ) and western blot analysis ( G ) detect SFMBT1 mRNA and protein in human colorectal epithelial cells CCD18C0 and CRC cell lines HCT-116, SW620 and SW480.

Product Details

PA5-22393

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 13 and 225 of SFMBT1 (Uniprot ID#Q9UHJ3) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.6 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.1M tris glycine, pH 7, with 20% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11152461

Product Specific Information

Recommended positive controls: NT2D1, IMR32, Jurkat, HeLa.

Predicted reactivity: Mouse (88%), Rat (87%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene shares high similarity with the Drosophila Scm (sex comb on midleg) gene. It encodes a protein which contains four malignant brain tumor repeat (mbt) domains and may be involved in antigen recognition.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SFMBT1 facilitates colon cancer cell metastasis and drug resistance combined with HMG20A.

Cell death discovery

Pan R,Yu D,Hu J,Yang X,Wang C,Zhang L,Xue P,Sun J,Zhang X,Cai W

PA5-22393 was used in Immunohistochemistry, Western Blot to indicate that the SFMBT1/HMG20A axis could be targeted to increase the resistance of CRC cells to 5-FU.

Mon May 16 00:00:00 EDT 2022

SFMBT1 facilitates colon cancer cell metastasis and drug resistance combined with HMG20A.

Cell death discovery

Pan R,Yu D,Hu J,Yang X,Wang C,Zhang L,Xue P,Sun J,Zhang X,Cai W

PA5-22393 was used in Immunohistochemistry, Western Blot to indicate that the SFMBT1/HMG20A axis could be targeted to increase the resistance of CRC cells to 5-FU.

Mon May 16 00:00:00 EDT 2022

SFMBT1 facilitates colon cancer cell metastasis and drug resistance combined with HMG20A.

Cell death discovery

Pan R,Yu D,Hu J,Yang X,Wang C,Zhang L,Xue P,Sun J,Zhang X,Cai W

PA5-22393 was used in Immunohistochemistry, Western Blot to indicate that the SFMBT1/HMG20A axis could be targeted to increase the resistance of CRC cells to 5-FU.

Mon May 16 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: hSFMBT; Renal ubiquitous protein 1; Scm-like with four MBT domains protein 1; Scm-related gene containing four mbt domains; Scm-related gene product containing four mbt domains

Gene Aliases: hSFMBT; RU1; SFMBT; SFMBT1

UniProt ID: (Human) Q9UHJ3

Entrez Gene ID: (Human) 51460

Molecular Function: chromatin/chromatin-binding, or -regulatory protein

Function(s)

transcription, DNA-templated spermatogenesis covalent chromatin modification cell differentiation negative regulation of transcription, DNA-templated negative regulation of muscle organ development

Process(es)

transcription corepressor activity protein binding histone binding

SFMBT1 Polyclonal Antibody

Product Details

PA5-22393

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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