Search
Invitrogen
SIN3A Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 8
SIN3A Antibody (PA1-870) in ICC/IF
Immunofluorescent analysis of Sin3A (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Sin3A polyclonal antibody (Product # PA1-870) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated ... View More
Product Details
PA1-870
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues M(1) K R R L D D Q E S P V Y A A Q Q R R(19) of mouse mSin3A.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-870 detects mSin3A from human and mouse tissues and cells.
PA1-870 has been successfully used in Western blot, ICC/IF ChIP, IHC-P and immunoprecipitation procedures. By Western blot, this antibody detects an ~150 kDa protein representing mSin3A from T47D cell extract.
PA1-870 immunogen is a synthetic peptide corresponding to residues M(1) K R R L D D Q E S P V Y A A Q Q R R(19) of mouse mSin3A.
Target Information
Mammalian Sin3 (mSin3) is a 150 kDa protein closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. There are two mammalian isoforms, mSin3A and mSin3B. Containing 4 paired amphipathic helix domains (PAH domains), mSin3A and mSin3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC1, HDAC2, RbAp46, the methyl CpG binding protein MeCP2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid and thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Histone deacetylase complex subunit Sin3a; Mammalian Sin3A; Paired amphipathic helix protein Sin3a; similar to yeast SIN3; SIN; SIN3 homolog A, transcription regulator; transcriptional co-repressor Sin3A; Transcriptional corepressor Sin3a; transcriptional regulator, SIN3 yeast homolog A; transcriptional regulator, SIN3A; unnamed protein product
Gene Aliases: AW553200; CHR15DELq24; DEL15Q24; Kiaa4126; mKIAA4126; mSin3A; Sin3; SIN3A; WITKOS
UniProt ID: (Human) Q96ST3, (Mouse) Q60520
Entrez Gene ID: (Human) 25942, (Mouse) 20466
DNA binding
chromatin binding
transcription corepressor activity
RNA binding
protein binding
macromolecular complex binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
transcription regulator inhibitor activity
transcription regulatory region sequence-specific DNA binding
RNA polymerase II activating transcription factor binding
RNA polymerase II repressing transcription factor binding
RNA polymerase II transcription corepressor activity
transcription factor activity, sequence-specific DNA binding
histone deacetylase activity
transcription factor binding
protein complex binding
protein deacetylase activity
chromatin/chromatin-binding, or -regulatory protein
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
activation of innate immune response
positive regulation of defense response to virus by host
hematopoietic progenitor cell differentiation
DNA replication
regulation of transcription, DNA-templated
protein localization
regulation of hormone levels
positive regulation of G2/M transition of mitotic cell cycle
cerebral cortex neuron differentiation
negative regulation of cell migration
negative regulation of transforming growth factor beta receptor signaling pathway
regulation of axon extension
heterochromatin assembly
negative regulation of circadian rhythm
negative regulation of apoptotic process
positive regulation of neuron differentiation
negative regulation of transcription, DNA-templated
rhythmic process
response to methylglyoxal
type I interferon signaling pathway
cellular response to glucose stimulus
cellular response to tert-butyl hydroperoxide
negative regulation of protein localization to nucleus
negative regulation of stem cell population maintenance
positive regulation of stem cell population maintenance
cellular response to dopamine
transcription, DNA-templated
transcription from RNA polymerase II promoter
protein deacetylation
aging
response to organonitrogen compound
histone deacetylation
positive regulation of chromatin silencing
cellular protein localization
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
positive regulation of transcription from RNA polymerase II promoter
negative regulation of histone H3-K27 acetylation
negative regulation of transcription regulatory region DNA binding
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support