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Invitrogen
SIX5 Polyclonal Antibody
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FIGURE: 1 / 2
SIX5 Antibody (PA5-101644) in ICC/IF
Immunofluorescent analysis of SIX5 in 3T3 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and SIX5 polyclonal antibody (Product # PA5-101644) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-101644
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SIX5(Accession Q8N196), corresponding to amino acid residues A611-A661.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total SIX5.
Target Information
Six5 (homeobox protein SIX5), also known as SIX5, BOR2 or DMAHP (DM locus-associated homeodomain protein), is a transcription factor that is expressed in various structures of the adult eye. Localized to the cytoplasm in early development and to the nucleus in the later stages of development, Six5 is involved in regulation of organogenesis and in maintenance of retinal formation. Six5 is able to bind the 5'-TCA[AG][AG]TTNC-3' DNA sequence found in the myogenin and IGFBP5 promoters and, through this binding, can control transcription of the associated mRNA. Six5 is regulated via association with DACH1 (dachshund homolog 1) and is co-activated by the EYA (eyes absent) proteins. Defects in the gene encoding Six5 are the cause of branchiooto- renal syndrome type 2 (BOR2), an autosomal disorder characterized by hearing loss, a deep overbite and myopia. Two isoforms exist due to alternative splicing events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DM locus-associated homeodomain protein; DM locus-associated homeodomain protein homolog; dystrophia myotonica-associated homeodomain protein; Homeobox protein SIX5; Sine oculis homeobox homolog 5; sine oculis-related homeobox 5 homolog
Gene Aliases: BOR2; DMAHP; MDMAHP; SIX5; TrexBF
UniProt ID: (Human) Q8N196, (Mouse) P70178
Entrez Gene ID: (Human) 147912, (Mouse) 20475
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
protein binding
sequence-specific DNA binding
homeodomain transcription factor
lens development in camera-type eye
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
spermatid development
cell proliferation
negative regulation of cell proliferation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
Leydig cell proliferation
negative regulation of skeletal muscle satellite cell proliferation
transcription, DNA-templated
multicellular organism development
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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