Western blot of SLAMF8 in HEK293 lysate was performed using 10ug protein in RIPA buffer. Lysates were probed with a SLAMF8 polyclonal antibody (Product# PA5-37956) at a dilution of 0.01ug/ml (Lane A) and with a MYC Tag antibody at a dilution of 1:1000 (Lane C). Lane B shows mock-transfected HEK293 cells probed with PA5-37956 at a dilution of 0.01ug/ml. All lysates were incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-HADRVGPETENP, from the C-terminus of SLAMF8|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.01-0.03 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.