|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Chicken / IgY|
|Immunogen||Purified recombinant SLC19A1 corresponding to the chromosome 21 gene (Human).|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA1-9553 detects SLC19A1 in human samples.
PA1-9553 has been successfully used in Western blot procedures. By Western blot, PA1-9553 detects a 65 kDa protein representing SLC19A1.
PA1-9553 immunogen is a purified recombinant SLC19A1 corresponding to the chromosome 21 gene (Human).
Transport of folate compounds into mammalian cells can occur via receptor-mediated (see MIM 136430) or carrier-mediated mechanisms. A functional coordination between these 2 mechanisms has been proposed to be the method of folate uptake in certain cell types. Methotrexate (MTX) is an antifolate chemotherapeutic agent that is actively transported by the carrier-mediated uptake system. RFC1 plays a role in maintaining intracellular concentrations of folate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Paradoxical impact of two folate receptors, FR¿ and RFC, in ovarian cancer: effect on cell proliferation, invasion and clinical outcome.
PA1-9553 was used in immunohistochemistry and western blot to study the expression and significance of the FRalpha and FRC folate receptors in ovarian cancer
|Siu MK,Kong DS,Chan HY,Wong ES,Ip PP,Jiang L,Ngan HY,Le XF,Cheung AN||PloS one (7:null)||2012|