Invitrogen

SLC25A26 Polyclonal Antibody

Product Image Gallery

SLC25A26 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of SLC25A26 in human cell line U-2 OS shows positivity in mitochondria. Samples were probed using a SLC25A26 Polyclonal Antibody (Product # PA5-55452).

SLC25A26 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of SLC25A26 in human testis using SLC25A26 Polyclonal Antibody (Product # PA5-55452) shows strong granular cytoplasmic positivity in a subset of cells in seminiferous ducts.

SLC25A26 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Fig. 3 CTB promoted accumulation of SLC25A26. A Western blots were used to detect the protein expressions of Ki67, HMGA1, and SLC25A26 in liver cancer tissues and adjacent tissues from five pairs of HCC patients (T: tumor; A: Adjacent); B , C Real-time PCR analyses of Ki67, SLC25A26 and senescent makers in HCC tumor and adjacent tissues; D Typical images of Ki67, HMGA1 and SLC25A26 immunohistochemical staining in HCC tumors and adjacent tissues (scale bar, 50 mum); E The mRNA level of SLC25A26 was detected by real-time PCR. F Western blot analysis was used to determine the expression of SLC25A26. Statistical significance for this graph, data are represented as mean +- S.D. ( n = 5); * P < 0.05 vs. control, ** P < 0.01 vs. control.

SLC25A26 Antibody in Western Blot (WB) — Fig. 7 CTB repressed tumorigenesis in vivo by regulating SLC25A26. HCC cell line Huh-7 was used to construct subcutaneous xenografts. A Representative images of BALB/c nude mice tumors stripped from different groups ( n = 6); B The body weight change curves of mice in each group during the whole experiment period ( n = 6); C Tumor volume growth curves of mice in each group throughout the experimental period ( n = 6); D Tumor weight was obtained after executing mice on the last day ( n = 6); E Real-time PCR analysis of p16, p21, HMGA1 mRNA levels in tumor tissues; F Western blot was used to analyze the protein expression of p16, p21, HMGA1 in tumor tissues. Representative blots were from three independent experiments; G Immunofluorescence analyses of senescent makers p16, p21, HMGA1 in the tumor tissue; H Elisa kit analyzes SAM levels in the tumor tissue; I Real-time PCR analyses of MAT2A, Ki67 mRNA levels in tumor tissues. J Western blot was used to analyze the protein expression of MAT2A, SLC25A26, Ki67 in tumor tissues. Representative blots were from three independent experiments; K Immunofluorescence analyses of MAT2A, SLC25A26, Ki67 in tumor tissues; Scale bars are 50 mum. Statistical significance for this graph: * P < 0.05 vs. control, ** P < 0.01 vs. control, # P < 0.05 vs. CTB (5 mg/kg) + shSLC25A26, ##P < 0.01 vs. CTB (5 mg/kg) + shSLC25A26.

Product Details

PA5-55452

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 1 publication 1 publication
Immunohistochemistry (IHC)	1:20-1:50	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human SLC25A26 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.05 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2647408

Product Specific Information

Immunogen sequence: SAGEVVACLI RVPSEVVKQR AQVSASTRTF QIFSNILYEE GIQGLYRGYK STVLREIPFS LVQFPLWESL KALWSWRQDH VVDSWQSAVC GAFAGGFAAA VTTPLDVAKT RITLAKAGSS TADGNVLSVL HGV

Highest antigen sequence identity to the following orthologs: Mouse - 89%, Rat - 89%.

Target Information

Mitochondrial carriers, including SLC25A26, are a family of transport proteins found mostly in the inner membranes of mitochondria. They shuttle metabolites and cofactors through the mitochondrial membrane.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Novel copper complex CTB regulates methionine cycle induced TERT hypomethylation to promote HCC cells senescence via mitochondrial SLC25A26.

Cell death & disease

Jin C,Li Y,Su Y,Guo Z,Wang X,Wang S,Zhang F,Zhang Z,Shao J,Zheng S

PA5-55452 was used in Immunohistochemistry to investigate the role of CTB in hepatocellular carcinoma cell senescence.

Sun Oct 11 00:00:00 EDT 2020

Novel copper complex CTB regulates methionine cycle induced TERT hypomethylation to promote HCC cells senescence via mitochondrial SLC25A26.

Cell death & disease

Jin C,Li Y,Su Y,Guo Z,Wang X,Wang S,Zhang F,Zhang Z,Shao J,Zheng S

PA5-55452 was used in Immunohistochemistry to investigate the role of CTB in hepatocellular carcinoma cell senescence.

Sun Oct 11 00:00:00 EDT 2020

Novel copper complex CTB regulates methionine cycle induced TERT hypomethylation to promote HCC cells senescence via mitochondrial SLC25A26.

Cell death & disease

Jin C,Li Y,Su Y,Guo Z,Wang X,Wang S,Zhang F,Zhang Z,Shao J,Zheng S

PA5-55452 was used in Immunohistochemistry to investigate the role of CTB in hepatocellular carcinoma cell senescence.

Sun Oct 11 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: Mitochondrial S-adenosylmethionine transporter; S-adenosylmethionine mitochondrial carrier protein; solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 26; solute carrier family 25 (S-adenosylmethionine carrier), member 26; Solute carrier family 25 member 26

Gene Aliases: COXPD28; SAMC; SLC25A26

UniProt ID: (Human) Q70HW3

Entrez Gene ID: (Human) 115286

Molecular Function: mitochondrial carrier protein