Alomone Labs, LTD

SLC6A8 Polyclonal Antibody

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Datasheet

Protocols

Questions & Answers

Tech Support

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SLC6A8 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Expression of creatine transporter in rat hippocampus and cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC6A8 Antibody (#AGT-008), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. Creatine transporter staining (green) in the hippocampal CA1 region is detected in apical dendrites (arrows). B. Creatine transporter immunoreactivity (green) in cortex is observed in soma and apical dendrites of neurons (arrows). Cell nuclei are stained with DAPI (blue).

SLC6A8 Antibody in Western Blot (WB) — Western blot analysis of mouse heart (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat brain (lanes 3 and 6)lysates: - 1-3. Anti-SLC6A8 Antibody (#AGT-008), (1:200).4-6. Anti-SLC6A8 Antibody , preincubated with SLC6A8 Blocking Peptide (#BLP-GT008).

Product Details

AGT-008-200UL

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200	-
Immunohistochemistry (Frozen) (IHC (F))	1:200	-

Product Specifications
Species Reactivity	Rat, Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	(C)RLAVPPRETWTRQMD, corresponding to amino acid residues 46-60 of rat creatine transporter, Extracellular, C-terminus View immunogen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.8 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 1% BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitution: 1 X 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

SLC6A8 (solute carrier family 6 member 8), also known as CT1 (creatine transporter 1), CRT or CRTR, is a 635 amino acid multi-pass plasma membrane protein that belongs to the sodium/neurotransporter (SNF) family. Expressed in a variety of tissues including kidney, skeletal muscle, heart, brain, prostate, testis and colon, SLC6A8 functions to transport creatine into and out of cells, specifically those found in brain and muscle tissue. Creatine, an organic acid, occurs naturally and is essential for supplying the energy needed for proper muscle and nerve function. Defects in the gene encoding SLC6A8 lead to cerebral creatine deficiency and are the cause of X-linked creatine deficiency syndrome, a rare disorder characterized by facial anomalies, seizures and mental retardation. Multiple isoforms of SLC6A8 exist due to alternative splicing events.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: choline transporter; CHOT1; creatine transporter 1; creatine transporter SLC6A8 variant D; creatine transporter, solute carrier family 6, member 8; CT1; MGC87396; Sodium- and chloride-dependent creatine transporter 1; solute carrier family 6 (neurotransmitter transporter), member 8; solute carrier family 6 (neurotransmitter transporter, creatine), member 8; Solute carrier family 6 member 8; solute carrier family 6, member 8

Gene Aliases: AA589632; CCDS1; CHOT1; CHT1; Creat; CRT; CRTR; CT1; CTR5; SLC6A8

UniProt ID: (Human) P48029, (Rat) P28570, (Mouse) Q8VBW1

Entrez Gene ID: (Human) 6535, (Rat) 50690, (Mouse) 102857

Molecular Function: primary active transporter