Invitrogen

SLC7A11 Polyclonal Antibody

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SLC7A11 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of SLC7A11 in the absorptive epithelia of small intestinal villi. Samples were incubated in SLC7A11 polyclonal antibody (Product # PA1-16893).

SLC7A11 Antibody in Western Blot (WB) — Western blot analysis of SLC7A11 in human HeLa cells. Samples were incubated in SLC7A11 polyclonal antibody (Product # PA1-16893) using a dilution of 2 µg/mL followed by an anti-rabbit HRP secondary antibody. Cells treated with and without 0.1 mM Diethyl Maleate for 24 hours was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. Detection: chemiluminescence. Note the increase in xCT expression with treatment.

SLC7A11 Antibody in Immunohistochemistry (IHC) — Published figure using SLC7A11 polyclonal antibody (Product # PA1-16893) in Immunohistochemistry

SLC7A11 Antibody in Western Blot, Immunoprecipitation (WB, IP) — FIGURE 4 Interactions between TRIM26 and SLC7A11. (A) Co-immunoprecipitation of TRIM26 and SLC7A11 in LX-2 cells. (B) Protein and mRNA expressions of TRIM26 and SLC7A11 in Vector- or oe TRIM26 plasmid-transfected LX-2 cells. (C) SLC7A11 ubiquitination in Vector-, oe TRIM26 plasmid, or oe C31S plasmid-transfected LX-2 cells. *** P < 0.001. ns: not significant.

Product Details

PA1-16893

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 6 publications 6 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	5 µg/mL	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Flow Cytometry (Flow)	2-3 µg/mL	-
Immunoprecipitation (IP)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide between amino acid 1-50 of the human XCT protein. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2286208

Product Specific Information

This antibody is not recommended for Immunoprecipitation.

Permeablization is recommended prior to performing Flow analysis.

Suggested positive control: antigen standard for SLC7A11 (transient overexpression lysate), human stomach protein or stomach tissue lysate.

Target Information

SLC7A11 (also known as XCT) is a member of a hetromeric, sodium-independent, anionic amino acid transport system. It is highly specific for cysteine and glutamate. SLC7A11 is a mediator of Kaposi sarcoma-associated herpes virus fusion and entry into cells. Increased expression of this protein in primary gliomas is associated with neuronal cell death.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transcriptome Profiles of IncRNA and mRNA Highlight the Role of Ferroptosis in Chronic Neuropathic Pain With Memory Impairment.

Frontiers in cell and developmental biology

Tang Y,Liu C,Zhu T,Chen H,Sun Y,Zhang X,Zhao Q,Wu J,Fei X,Ye S,Chen C

Published figure using SLC7A11 polyclonal antibody (Product # PA1-16893) in Western Blot

Sat Jul 16 00:00:00 EDT 2022

Overexpression of Cystine/Glutamate Antiporter xCT Correlates with Nutrient Flexibility and ZEB1 Expression in Highly Clonogenic Glioblastoma Stem-like Cells (GSCs).

Cancers

Koch K,Hartmann R,Suwala AK,Rios DH,Kamp MA,Sabel M,Steiger HJ,Willbold D,Sharma A,Kahlert UD,Maciaczyk J

PA1-16893 was used in Western Blot to study the metabolic dependencies of glioblastoma stem-like cells with high-resolution proton nuclear magnetic resonance spectroscopy.

Mon Nov 29 00:00:00 EST 2021

Overexpression of Cystine/Glutamate Antiporter xCT Correlates with Nutrient Flexibility and ZEB1 Expression in Highly Clonogenic Glioblastoma Stem-like Cells (GSCs).

Cancers

Koch K,Hartmann R,Suwala AK,Rios DH,Kamp MA,Sabel M,Steiger HJ,Willbold D,Sharma A,Kahlert UD,Maciaczyk J

PA1-16893 was used in Western Blot to study the metabolic dependencies of glioblastoma stem-like cells with high-resolution proton nuclear magnetic resonance spectroscopy.

Mon Nov 29 00:00:00 EST 2021

TRIM26 Induces Ferroptosis to Inhibit Hepatic Stellate Cell Activation and Mitigate Liver Fibrosis Through Mediating SLC7A11 Ubiquitination.

Frontiers in cell and developmental biology

Zhu Y,Zhang C,Huang M,Lin J,Fan X,Ni T

Published figure using SLC7A11 polyclonal antibody (Product # PA1-16893) in Immunoprecipitation

Wed Apr 21 00:00:00 EDT 2021

TRIM26 Induces Ferroptosis to Inhibit Hepatic Stellate Cell Activation and Mitigate Liver Fibrosis Through Mediating SLC7A11 Ubiquitination.

Frontiers in cell and developmental biology

Zhu Y,Zhang C,Huang M,Lin J,Fan X,Ni T

Published figure using SLC7A11 polyclonal antibody (Product # PA1-16893) in Immunoprecipitation

Wed Apr 21 00:00:00 EDT 2021

Mitochondrial fusion supports increased oxidative phosphorylation during cell proliferation.

eLife

Yao CH,Wang R,Wang Y,Kung CP,Weber JD,Patti GJ

PA1-16893 was used in Western Blotting to reveal that cell proliferation requires increased OXPHOS as supported by mitochondrial fusion.

Tue Jan 29 00:00:00 EST 2019

Mitochondrial fusion supports increased oxidative phosphorylation during cell proliferation.

eLife

Yao CH,Wang R,Wang Y,Kung CP,Weber JD,Patti GJ

PA1-16893 was used in Western Blotting to reveal that cell proliferation requires increased OXPHOS as supported by mitochondrial fusion.

Tue Jan 29 00:00:00 EST 2019

Participation of xCT in melanoma cell proliferation in vitro and tumorigenesis in vivo.

Oncogenesis

Shin SS,Jeong BS,Wall BA,Li J,Shan NL,Wen Y,Goydos JS,Chen S

PA1-16893 was used in Western Blotting to show that xCT may be utilised as a marker to monitor patients undergoing riluzole-based chemotherapies.

Wed Nov 14 00:00:00 EST 2018

Participation of xCT in melanoma cell proliferation in vitro and tumorigenesis in vivo.

Oncogenesis

Shin SS,Jeong BS,Wall BA,Li J,Shan NL,Wen Y,Goydos JS,Chen S

PA1-16893 was used in Western Blotting to show that xCT may be utilised as a marker to monitor patients undergoing riluzole-based chemotherapies.

Wed Nov 14 00:00:00 EST 2018

Participation of xCT in melanoma cell proliferation in vitro and tumorigenesis in vivo.

Oncogenesis

Shin SS,Jeong BS,Wall BA,Li J,Shan NL,Wen Y,Goydos JS,Chen S

PA1-16893 was used in Western Blotting to show that xCT may be utilised as a marker to monitor patients undergoing riluzole-based chemotherapies.

Wed Nov 14 00:00:00 EST 2018

Comparative analysis of antibodies to xCT (Slc7a11): Forewarned is forearmed.

The Journal of comparative neurology

Van Liefferinge J,Bentea E,Demuyser T,Albertini G,Follin-Arbelet V,Holmseth S,Merckx E,Sato H,Aerts JL,Smolders I,Arckens L,Danbolt NC,Massie A

Published figure using SLC7A11 polyclonal antibody (Product # PA1-16893) in Western Blot

Fri Apr 01 00:00:00 EDT 2016

Comparative analysis of antibodies to xCT (Slc7a11): Forewarned is forearmed.

The Journal of comparative neurology

Van Liefferinge J,Bentea E,Demuyser T,Albertini G,Follin-Arbelet V,Holmseth S,Merckx E,Sato H,Aerts JL,Smolders I,Arckens L,Danbolt NC,Massie A

Published figure using SLC7A11 polyclonal antibody (Product # PA1-16893) in Western Blot

Fri Apr 01 00:00:00 EDT 2016

Reduced expression of miRNA-27a modulates cisplatin resistance in bladder cancer by targeting the cystine/glutamate exchanger SLC7A11.

Clinical cancer research : an official journal of the American Association for Cancer Research

Drayton RM,Dudziec E,Peter S,Bertz S,Hartmann A,Bryant HE,Catto JW

PA1-16893 was used in immunohistochemistry to study the role of SLC7A11 in the mechanism by which miRNA-27a expression levels modulate bladder cancer cisplatin resistance

Tue Apr 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Amino acid transport system xc-; Calcium channel blocker resistance protein CCBR1; cysteine/glutamate transporter; Cystine/glutamate transporter; sodium independent anionic amino acid transport system; solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11; solute carrier family 7 (cationic amino acid transporter, y+ system), member 11; Solute carrier family 7 member 11; solute carrier family 7, (cationic amino acid transporter, y+ system) member 11; xCT

Gene Aliases: 9930009M05Rik; AI451155; CCBR1; SLC7A11; sut; xCT

UniProt ID: (Human) Q9UPY5, (Mouse) Q9WTR6

Entrez Gene ID: (Human) 23657, (Mouse) 26570, (Rat) 310392

Molecular Function: transporter