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Immunofluorescent analysis of SLU7 showing staining in the cytoplasm and nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a SLU7 polyclonal antibody (Product # PA5-28513) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 349 and 586 of Human SLU7|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28513 targets SLU7 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-28513 immunogen is recombinant fragment corresponding to a region within amino acids 349 and 586 of Human SLU7.
Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
9G8; pre-mRNA-splicing factor SLU7; SLU7 splicing factor homolog; splicing factor; step II splicing factor SLU7; zinc knuckle motif containing
9G8; hSlu7; SLU7