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SMARCA2 Antibody (PA5-78170) in ICC/IF
SMARCA2 Polyclonal Antibody detects SMARCA2 protein at nucleolus by immunofluorescent analysis. Sample: DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMARCA2 stained by SMARCA2 Polyclonal Antibody (Product # PA5-78170) diluted at 1:500. Red: Tau, a marker, stained by Phospho-Tau (Ser262) Polyclonal Antibody [GT287]diluted at 1:500.
Product Details
PA5-78170
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SMARCA2. The exact sequence is proprietary.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.89 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2736632
Product Specific Information
Positive Control: 293T, A431, HeLa, HepG2
Predicted Reactivity: Chicken (100%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
SMARCA2 encodes a protein that is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Two transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP-dependent helicase SMARCA2; BAF190B; BRG1-associated factor 190B; FLJ36757; global transcription activator homologous sequence; hBRM; MGC74511; Probable global transcription activator SNF2L2; Protein brahma homolog; SNF2-alpha; SNF2/SWI2-like protein 2; subunit of the SWI/SNF chromatin remodeling complex; sucrose nonfermenting 2-like protein 2; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2
Gene Aliases: 2610209L14Rik; BAF190; BAF190B; brahma; BRM; hBRM; hSNF2a; NCBRS; SMARCA2; SNF2; SNF2A; SNF2alpha; SNF2L2; SNF2LA; Sth1p; SWI2
UniProt ID: (Human) P51531, (Mouse) Q6DIC0
Entrez Gene ID: (Human) 6595, (Mouse) 67155
Molecular Function:
DNA helicase
negative regulation of transcription from RNA polymerase II promoter
chromatin remodeling
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
spermatid development
nervous system development
negative regulation of cell proliferation
negative regulation of cell growth
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
chromatin organization
nucleosome assembly
transcription from RNA polymerase II promoter
aortic smooth muscle cell differentiation
RNA polymerase II transcription coactivator activity
chromatin binding
transcription coactivator activity
helicase activity
protein binding
ATP binding
DNA-dependent ATPase activity
histone binding
transcription regulatory region DNA binding
nucleotide binding
DNA binding
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
ATPase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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