Invitrogen

SMARCC1 Polyclonal Antibody

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Datasheet

Protocols

Questions & Answers

Tech Support

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SMARCC1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of SMARCC1 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SMARCC1 Polyclonal Antibody (Product # PA5-30174) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

SMARCC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — SMARCC1 Polyclonal Antibody detects SMARCC1 protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. SMARCC1 Polyclonal Antibody (Product # PA5-30174) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

SMARCC1 Antibody in Western Blot (WB) — Western blot was performed using Anti-SMARCC1 Polyclonal Antibody (Product # PA5-30174) and a 160kDa band corresponding to SMARCC1 was observed in all the tested cell models. Modified whole cell lysate (1% SDS) (30ug lysate) of A-431 (Lane 1), U-2 OS (Lane 2), A549 (Lane 3), HeLa (Lane 4), K-562 (Lane 5), Jurkat (Lane 6) and MOLT-4 (Lane 7) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by wet transfer. The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

SMARCC1 Antibody in Immunoprecipitation (IP) — SMARCC1 antibody immunoprecipitates SMARCC1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 µg whole cell lysate/extract of SMARCC1 protein expressing 293T cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of SMARCC1 by 2.5 µg of SMARCC1 antibody (Product # PA5-30174) 5% SDS-PAGE The immunoprecipitated SMARCC1 protein was detected by SMARCC1 antibody (Product # PA5-30174) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.

SMARCC1 Antibody in ChIP Assay (ChIP) — Chromatin Immunoprecipitation (ChIP) assay of endogenous SMARCC1 protein using Anti-SMARCC1 Antibody: ChIP was performed using Anti-SMARCC1 Rabbit Polyclonal Antibody (Product # PA5-30174, 5 µg) on sheared chromatin from HeLa cells using the MAGnify ChIP System kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to pS2 and CCND1 promoters and SAT2 satellite repeats. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

Product Details

PA5-30174

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:20,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunoprecipitation (IP)	1:100-1:500	-
ChIP assay (ChIP)	2.5 µg/10^6 cells	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 808 and 1092 of Human SMARCC1 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	2.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2547648

Product Specific Information

Recommended positive controls: HeLa, HepG2, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12.

Predicted reactivity: Mouse (96%), Rat (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BAF155; BRG1-associated factor 155; chromatin remodeling complex BAF155 subunit; mammalian chromatin remodeling complex BRG1-associated factor 155; SWI/SNF complex 155 kDa subunit; SWI/SNF complex subunit SMARCC1; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1; SWI3-related protein

Gene Aliases: AI115498; BAF155; CRACC1; Rsc8; SMARCC1; SRG3; SWI3

UniProt ID: (Human) Q92922, (Mouse) P97496

Entrez Gene ID: (Human) 6599, (Rat) 301020, (Mouse) 20588

Molecular Function: chromatin/chromatin-binding, or -regulatory protein