Invitrogen

SMO Polyclonal Antibody

3 Published Figures
1 Reference
View all (29) SMO antibodies

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Datasheet
Protocols
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Datasheet
Protocols
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Product Details

PA5-76145

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein of human SMO.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2719872

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Target Information

Smoothened drosophila homolog (SMOH) is a serpentine protein belonging to the G-protein coupled receptor Fz/Smo family with 787 aa, seven transmembrane G protein-coupled receptors with 4 glycosylation sites and a putative extracellular amino terminus. Smoothened activated by b-Arrestin 2 and GRK2 acts as a receptor for Shh, the Sonic hedgehog gene product, and is a positive regulator of hedgehog signaling pathway along with the smoothened inhibitor patched protein (PTCH) and regulates vertebrate developmental processes, cellular proliferation and differentiation by activating transcription factor Gli. Smoothened mutants are known to cause sporadic basal cell carcinoma of the skin. It is known to express widely in heart and brain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: bent body; frizzled family member 11; HGNC:11119; Protein Gx; Protein SMOH; Protein smoothened; seven transmembrane helix receptor; smoothened homolog; smoothened, frizzled family receptor; smoothened, seven transmembrane spanning receptor

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Gene Aliases: bnb; E130215L21Rik; FZD11; Gx; SMO; SMOH; smoothened

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UniProt ID: (Human) Q99835, (Mouse) P56726

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Entrez Gene ID: (Human) 6608, (Mouse) 319757

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Function(s)
G-protein coupled receptor activity patched binding protein binding drug binding Wnt-protein binding Wnt-activated receptor activity signal transducer activity transmembrane signaling receptor activity transmembrane signal receptor
Process(es)
negative regulation of transcription from RNA polymerase II promoter vasculogenesis osteoblast differentiation in utero embryonic development cell fate specification neural crest cell migration heart looping positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation determination of left/right asymmetry in lateral mesoderm type B pancreatic cell development G-protein coupled receptor signaling pathway smoothened signaling pathway positive regulation of hh target transcription factor activity ventral midline determination midgut development anterior/posterior pattern specification positive regulation of gene expression negative regulation of gene expression dentate gyrus development cerebellar cortex morphogenesis thalamus development dorsal/ventral neural tube patterning smoothened signaling pathway involved in ventral spinal cord patterning smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation central nervous system neuron differentiation cerebral cortex development negative regulation of epithelial cell differentiation hair follicle morphogenesis protein localization to nucleus multicellular organism growth non-canonical Wnt signaling pathway positive regulation of multicellular organism growth positive regulation of protein import into nucleus odontogenesis of dentin-containing tooth negative regulation of apoptotic process negative regulation of DNA binding positive regulation of smoothened signaling pathway positive regulation of transcription from RNA polymerase II promoter positive regulation of organ growth astrocyte activation skeletal muscle fiber development forebrain morphogenesis homeostasis of number of cells within a tissue positive regulation of epithelial cell proliferation protein stabilization myoblast migration negative regulation of hair follicle development canonical Wnt signaling pathway detection of cell density by contact stimulus involved in contact inhibition atrial septum morphogenesis mammary gland epithelial cell differentiation epithelial-mesenchymal cell signaling somite development pancreas morphogenesis left/right axis specification cellular response to cholesterol renal system development mesenchymal to epithelial transition involved in metanephric renal vesicle formation positive regulation of branching involved in ureteric bud morphogenesis regulation of stem cell population maintenance regulation of heart morphogenesis ossification heart morphogenesis signal transduction cell surface receptor signaling pathway multicellular organism development determination of left/right symmetry pattern specification process central nervous system development positive regulation of cell proliferation dorsal/ventral pattern formation regulation of gene expression cell development digestive tract development embryonic organ development developmental growth axon extension involved in axon guidance
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