Proteintech

SMYD3 Polyclonal Antibody

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SMYD3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 12011-1-AP ( SMYD3 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

SMYD3 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 12011-1-AP (SMYD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SMYD3 Antibody in Immunoprecipitation (IP) — IP result of anti-SMYD3 (IP:12011-1-AP, 4ug; Detection:12011-1-AP 1:500) with COLO 320 cells lysate 1280ug.

Product Details

12011-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SMYD3 Fusion Protein Ag2624 (60-428 aa encoded by BC031010) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.27 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MRCSQCRVA KYCSAKCQKK AWPDHKRECK CLKSCKPRYP PDSVRLLGRV VFKLMDGAPS ESEKLYSFYD LESNINKLTE DRKEGLRQLV MTFQHFMREE IQDASQLPPA FDLFEAFAKV ICNSFTICNA EMQEVGVGLY PSISLLNHSC DPNCSIVFNG PHLLLRAVRD IEVGEELTIC YLDMLMTSEE RRKQLRDQYC FECDCFRCQT QDKDADMLTG DEQVWKEVQE SLKKIEELKA HWKWEQVLAM CQAIISSNSE RLPDINIYQL KVLDCAMDAC INLGLLEEAL FYGTRTMEPY RIFFPGSHPV RGVQVMKVGK LQLHQGMFPQ AMKNLRLAFD IMRVTHGREH SLIEDLILLL EECDANIRAS (60-428 aa encoded by BC031010)

Target Information

SMYD3 (histone-lysine N-methyltransferase) methylates Lys-4 of histone H3 and Lys-5 of histone H4. It induces di- and tri-methylation. SMYD3 plays an important role in transcriptional activation as a member of an RNA polymerase complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Liu X,Wang J,Gao L,Liu H,Liu C

Tue Mar 06 00:00:00 EST 2018

Effects of SMYD3 over-expression on cell cycle acceleration and cell proliferation in MDA-MB-231 human breast cancer cells.

Medical oncology (Northwood, London, England)

Ren TN,Wang JS,He YM,Xu CL,Wang SZ,Xi T

Thu Dec 01 00:00:00 EST 2011

Histone methylation is a critical regulator of the abnormal expression of POU5F1 and RASSF1A in testis cancer cell lines.

International journal of andrology

Lambrot R,Kimmins S

Fri Apr 01 00:00:00 EDT 2011

miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma.

Carcinogenesis

Furuta M,Kozaki KI,Tanaka S,Arii S,Imoto I,Inazawa J

Sat May 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: bA74P14.1 (novel protein); Histone-lysine N-methyltransferase SMYD3; SET and MYND domain-containing protein 3; Zinc finger MYND domain-containing protein 1; zinc finger protein, subfamily 3A (MYND domain containing), 1; zinc finger, MYND domain containing 1

Gene Aliases: 2410008A19Rik; bA74P14.1; KMT3E; SMYD3; ZMYND1; ZNFN3A1

UniProt ID: (Human) Q9H7B4, (Mouse) Q9CWR2

Entrez Gene ID: (Human) 64754, (Mouse) 69726, (Rat) 498295

Molecular Function: histone modifying enzyme