Proteintech

SNAPIN Polyclonal Antibody

Product Image Gallery

SNAPIN Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of HepG2 cells using 10055-1-AP (SNAPIN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

SNAPIN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis tissue slide using 10055-1-AP ( SNAPIN Antibody) at dilution of 1:200 (under 40x lens).

SNAPIN Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10055-1-AP (SNAPIN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SNAPIN Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug SNAPIN antibody (10055-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

SNAPIN Antibody in Immunoprecipitation (IP) — IP result of anti-SNAPIN (IP:10055-1-AP, 5ug; Detection:10055-1-AP 1:300) with mouse brain tissue lysate 4000ug.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunocytochemistry (ICC)

1:20-1:200

Immunofluorescence (IF)

1:20-1:200

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:200-1:1000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SNAPIN Fusion Protein Ag0101

Conjugate

Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody can recognize the 15-18 kDa (Monomer) and 30-36 kDa (Dimer) forms of SNAPIN.

Target Information

The protein encoded by this gene is a coiled-coil-forming protein that associates with the SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) complex of proteins and the BLOC-1 (biogenesis of lysosome-related organelles) complex. Biochemical studies have identified additional binding partners. As part of the SNARE complex, it is required for vesicle docking and fusion and regulates neurotransmitter release. The BLOC-1 complex is required for the biogenesis of specialized organelles such as melanosomes and platelet dense granules. Mutations in gene products that form the BLOC-1 complex have been identified in mouse strains that are models of Hermansky-Pudlak syndrome. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: biogenesis of lysosomal organelles complex-1, subunit 7; Biogenesis of lysosome-related organelles complex 1 subunit 7; BLOC-1 subunit 7; SNAP-25-binding protein; SNAP-associated protein; SNARE associated protein snapin; SNARE-associated protein Snapin; synaptosomal-associated protein 25 binding protein; Synaptosomal-associated protein 25-binding protein; synaptosomal-associated protein, 25 kDa, binding protein

Gene Aliases: 25kDa; AA407989; AV026596; BLOC1S7; BLOS7; BORCS3; SNAP25BP; SNAPAP; SNAPIN

UniProt ID: (Human) O95295, (Mouse) Q9Z266, (Rat) P60192

Entrez Gene ID: (Human) 23557, (Mouse) 20615, (Rat) 295217

Function(s)