Proteintech

SNAPIN Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

SNAPIN Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells using 10055-1-AP (SNAPIN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

SNAPIN Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis tissue slide using 10055-1-AP ( SNAPIN Antibody) at dilution of 1:200 (under 40x lens).

SNAPIN Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10055-1-AP (SNAPIN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SNAPIN Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug SNAPIN antibody (10055-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

SNAPIN Antibody in Immunoprecipitation (IP) — IP result of anti-SNAPIN (IP:10055-1-AP, 5ug; Detection:10055-1-AP 1:300) with mouse brain tissue lysate 4000ug.

Product Details

10055-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200-1:1,000	View 5 publications 5 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 1 publication 1 publication
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SNAPIN Fusion Protein Ag0101 (1-136 aa encoded by BC000761) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.35 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody can recognize the 15-18 kDa (Monomer) and 30-36 kDa (Dimer) forms of SNAPIN.

Immunogen sequence: MAGAGSAAV SGAGTPVAGP TGRDLFAEGL LEFLRPAVQQ LDSHVHAVRE SQVELREQID NLATELCRIN EDQKVALDLD PYVKKLLNAR RRVVLVNNIL QNAQERLRRL NHSVAKETAR RRAMLDSGIY PPGSPGK (1-136 aa encoded by BC000761)

Target Information

The protein encoded by this gene is a coiled-coil-forming protein that associates with the SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) complex of proteins and the BLOC-1 (biogenesis of lysosome-related organelles) complex. Biochemical studies have identified additional binding partners. As part of the SNARE complex, it is required for vesicle docking and fusion and regulates neurotransmitter release. The BLOC-1 complex is required for the biogenesis of specialized organelles such as melanosomes and platelet dense granules. Mutations in gene products that form the BLOC-1 complex have been identified in mouse strains that are models of Hermansky-Pudlak syndrome. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LAMTOR/Ragulator is a negative regulator of Arl8b- and BORC-dependent late endosomal positioning.

The Journal of cell biology

Filipek PA,de Araujo MEG,Vogel GF,De Smet CH,Eberharter D,Rebsamen M,Rudashevskaya EL,Kremser L,Yordanov T,Tschaikner P,Fürnrohr BG,Lechner S,Dunzendorfer-Matt T,Scheffzek K,Bennett KL,Superti-Furga G,Lindner HH,Stasyk T,Huber LA

Mon Dec 04 00:00:00 EST 2017

SNAPIN is critical for lysosomal acidification and autophagosome maturation in macrophages.

Autophagy

Shi B,Huang QQ,Birkett R,Doyle R,Dorfleutner A,Stehlik C,He C,Pope RM

Wed Feb 01 00:00:00 EST 2017

SNAPIN is critical for lysosomal acidification and autophagosome maturation in macrophages.

Autophagy

Shi B,Huang QQ,Birkett R,Doyle R,Dorfleutner A,Stehlik C,He C,Pope RM

Wed Feb 01 00:00:00 EST 2017

Annexin A1 complex mediates oxytocin vesicle transport.

Journal of neuroendocrinology

Makani V,Sultana R,Sie KS,Orjiako D,Tatangelo M,Dowling A,Cai J,Pierce W,Butterfield DA,Hill J,Park J

Sun Dec 01 00:00:00 EST 2013

A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome.

Skeletal muscle

Blandin G,Marchand S,Charton K,Danièle N,Gicquel E,Boucheteil JB,Bentaib A,Barrault L,Stockholm D,Bartoli M,Richard I

Fri Feb 15 00:00:00 EST 2013

A BLOC-1 mutation screen reveals a novel BLOC1S3 mutation in Hermansky-Pudlak Syndrome type 8.

Pigment cell & melanoma research

Cullinane AR,Curry JA,Golas G,Pan J,Carmona-Rivera C,Hess RA,White JG,Huizing M,Gahl WA

Sat Sep 01 00:00:00 EDT 2012

A BLOC-1 mutation screen reveals that PLDN is mutated in Hermansky-Pudlak Syndrome type 9.

American journal of human genetics

Cullinane AR,Curry JA,Carmona-Rivera C,Summers CG,Ciccone C,Cardillo ND,Dorward H,Hess RA,White JG,Adams D,Huizing M,Gahl WA

Fri Jun 10 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: biogenesis of lysosomal organelles complex-1, subunit 7; Biogenesis of lysosome-related organelles complex 1 subunit 7; BLOC-1 subunit 7; SNAP-25-binding protein; SNAP-associated protein; SNARE associated protein snapin; SNARE-associated protein Snapin; synaptosomal-associated protein 25 binding protein; Synaptosomal-associated protein 25-binding protein; synaptosomal-associated protein, 25 kDa, binding protein

Gene Aliases: 25kDa; AA407989; AV026596; BLOC1S7; BLOS7; BORCS3; SNAP25BP; SNAPAP; SNAPIN

UniProt ID: (Human) O95295, (Mouse) Q9Z266, (Rat) P60192

Entrez Gene ID: (Human) 23557, (Mouse) 20615, (Rat) 295217

Function(s)