|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide corresponding to a portion of human manganese superoxide dismutase encoded within exon 3.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris phosphate/tris citrate, pH 7-8|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:4000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-20324 detects MnSOD in rat samples.
PA1-20324 has been successfully used in Western blot procedures.
The PA1-20324 immunogen is a synthetic peptide corresponding to a portion of human manganese superoxide dismutase encoded within exon 3.
Superoxide dismutase (SOD) is responsible for the elimination of cytotoxic active oxygen by catalyzing the dismutation of the superoxide radical to oxygen and hydrogen peroxide. There are three SOD isoenzymes in mammalian cells. They are: extracellular SOD (EC SOD), copper and zinc-containing SOD (Cu/Zn SOD) and manganese-containing SOD (Mn SOD). The Cu/Zn form contains Cu and Zn ions and exists as a 32 kDa dimer in the cytosol. Mn SOD is an 80 kDa tetramer that contains Mn ion and resides in the mitochondrial matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.