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Invitrogen
SOS1 Polyclonal Antibody
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FIGURE: 1 / 1
SOS1 Antibody (PA5-96239) in WB
Western blot analysis of SOS1 in extracts of various cell lines. Samples were incubated with SOS1 Polyclonal antibody (Product # PA5-96239) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-96239
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1034-1333 of human SOS1 (NP_0056242)
Conjugate
Form
Liquid
Concentration
1.14 mg/mL
Amount
114 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: PLKSPGVRPS NPRPGTMRHP TPLQQEPRKI SYSRIPESET ESTASAPNSP RTPLTPPPAS GASSTTDVCS VFDSDHSSPF HSSNDTVFIQ VTLPHGPRSA SVSSISLTKG TDEVPVPPPV PPRRRPESAP AESSPSKIMS KHLDSPPAIP PRQPTSKAYS PRYSISDRTS ISDPPESPPL LPPREPVRTP DVFSSSPLHL QPPPLGKKSD HGNAFFPNSP SPFTPPPPQT PSPHGTRRHL PSPPLTQEVD LHSIAGPPVP PRQSTSQHIP KLPPKTYKRE HTHPSMHRDG PPLLENAHSS; Positive Samples: HeLa, Mouse liver, Rat brain, Rat liver
Target Information
Son of sevenless (SOS) is a guanine nucleotide exchange factor that activates Ras in response to growth factor stimulation. Sos1, and its closely related Sos2 paralog, are comprised of an N-terminal histone-binding domain, a RacGEF catalytic (DH) domain juxtaposed with a PH domain, a Ras exchanger motif (REM) domain, a RasGEF catalytic domain, and a C-terminal proline-rich region. Sos1 catalyzes the GDP-GTP exchange within the membrane-bound GTPase Ras and thereby switches on a key signaling circuit that involves the activation of MAPK cascade central to cellular proliferation, survival and differentiation. Growth factor stimulation rapidly induces the phosphorylation of Sos on multiple serine and threonine sites. Phosphorylation of residues within the Sos C-terminus by the MAP/ERK kinase disrupts the Grb2-Sos complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G GINGF; gingival fibromatosis; gingival fibromatosis, hereditary, 1; guanine nucleotide exchange factor; guanine nucleotide releasing factor; ras guanine exchange factor; Son of sevenless homolog 1; son-of-sevenless 1; SOS-1; unnamed protein product
Gene Aliases: 4430401P03Rik; 9630010N06; AI449023; GF1; GGF1; GINGF; HGF; NS4; SOS-1; SOS1
UniProt ID: (Human) Q07889, (Mouse) Q62245
Entrez Gene ID: (Human) 6654, (Rat) 313845, (Mouse) 20662
guanyl-nucleotide exchange factor activity
GTPase activator activity
epidermal growth factor receptor binding
protein binding
SH3 domain binding
protein kinase binding
protein heterodimerization activity
molecular condensate scaffold activity
DNA binding
Ras guanyl-nucleotide exchange factor activity
Rho guanyl-nucleotide exchange factor activity
guanyl-nucleotide exchange factor
B cell homeostasis
hair follicle development
lymphocyte homeostasis
response to ischemia
heart morphogenesis
cardiac atrium morphogenesis
pericardium morphogenesis
regulation of transcription from RNA polymerase II promoter
signal transduction
epidermal growth factor receptor signaling pathway
small GTPase mediated signal transduction
Ras protein signal transduction
vitellogenesis
axon guidance
insulin receptor signaling pathway
fibroblast growth factor receptor signaling pathway
Schwann cell development
cytokine-mediated signaling pathway
regulation of T cell differentiation in thymus
multicellular organism growth
Fc-epsilon receptor signaling pathway
T cell activation
regulation of cell proliferation
regulation of T cell proliferation
myelination
positive regulation of epidermal growth factor receptor signaling pathway
insulin-like growth factor receptor signaling pathway
neurotrophin TRK receptor signaling pathway
blood vessel morphogenesis
B cell receptor signaling pathway
leukocyte migration
positive regulation of small GTPase mediated signal transduction
palate development
eyelid development in camera-type eye
heart trabecula morphogenesis
midbrain morphogenesis
regulation of pro-B cell differentiation
regulation of Rho protein signal transduction
positive regulation of GTPase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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