Western blot analysis of SOX1 in human brain lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse brain lysate using a SOX1 polyclonal antibody (Product # PA5-23370) at 0.5 ug/ml, 0.5 ug/ml and 2 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody.
|Tested species reactivity||Dog, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (366 SRLHSLPQHYQGAGA 380) of SOX1 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
SOX1, also known as SRY (sex determining region Y)-box 1, is a transcription factor belonging to the SRY-related HMG box family of transcription factors with a HMG box DNA-binding domain. SOX1 works through multiple independent pathways to promote neuronal cell fate determination and differentiation. This nuclear protein acts as a modulator of LINE retroposons promoter activity and is also involved in the development of the central nervous system, mainly neural and lens development. Transcriptional activator SOX1 binds directly to the HES1 promoter and suppresses HES1 transcription, thus attenuating Notch signaling. It also binds to beta-catenin and suppresses beta-catenin-mediated TCF/LEF signaling, thus potentially attenuating the WNT signaling pathway. Expression of SOX1 is seen mostly in embryonic tissues and moderately in brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.