Invitrogen

SOX18 Polyclonal Antibody

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View all (9) SOX18 antibodies

Questions & Answers

Questions & Answers

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SOX18 Antibody in Western Blot (WB) — Western Blot analysis of SOX18 was performed by loading Jurkat cells. Proteins were transferred to a membrane and probed with a SOX18 Polyclonal Antibody (Product # PA1-24474) at a dilution of 2.0 µg/mL.

SOX18 Antibody in ChIP assay, Gel Shift (ChIP, GS) — Figure 4 SOX18 binds to the proximal site in the MMP7 promoter in vitro and in vivo . A, Electrophoretic mobility shift assay (EMSA). Binding of SOX18 to its proximal site in the MMP7 promoter was analyzed using nuclear extracts from HUVEC. Specificity of binding was demonstrated by competition experiments using the same (wt), a mutated (mut) or an unrelated (unrel) oligonucleotide as indicated, the specific complex is indicated by an arrow. B, The same site was analyzed by chromatin immunoprecipitation in HUVEC. Lane 1-3: different alpha-Sox18 antibodies (SantaCruz, Thermo, Chemicon, respectively) as described in Materials and Methods , Ig: IgG control antibody; inp: input, M: 100 bp marker. The 120 bp PCR fragments obtained after amplification ofDNA precipitated by the anti-SOX18 antibodies and input DNA are marked by an arrow.

Product Details

PA1-24474

Applications	Tested Dilution	Publications
Western Blot (WB)	0.2-2.5 µg/mL	View 1 publication 1 publication
ChIP assay (ChIP)	-	View 1 publication 1 publication
Gel Shift (GS)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide region of human SOX18.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	IgG fraction
Storage buffer	PBS with 2% sucrose
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2195668

Product Specific Information

Recommended positive controls: Jurkat.

Predicted reactivity: Cow: 92%; Dog: 100%; Guinea Pig: 100%; Mouse: 85%; Rat: 85%.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

SOX18 a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. This protein plays a role in hair, blood vessel, and lymphatic vessel development. Mutations in this gene have been associated with recessive and dominant forms of hypotrichosis-lymphedema-telangiectasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TNFα-induced down-regulation of Sox18 in endothelial cells is dependent on NF-κB.

Biochemical and biophysical research communications

Basílio J,Hoeth M,Holper-Schichl YM,Resch U,Mayer H,Hofer-Warbinek R,de Martin R

PA1-24474 was used in western blot to study the role of NFkB in the mechanism by which TNF-alpha downregulates endothelial Sox18 expression

Fri Dec 13 00:00:00 EST 2013

The transcription factor SOX18 regulates the expression of matrix metalloproteinase 7 and guidance molecules in human endothelial cells.

PloS one

Hoeth M,Niederleithner H,Hofer-Warbinek R,Bilban M,Mayer H,Resch U,Lemberger C,Wagner O,Hofer E,Petzelbauer P,de Martin R

PA1-24474 was used in ChIP assay to study the target genes of transcription factor SOX18 in human endothelial cells

Mon Jun 04 00:00:00 EDT 2012

The transcription factor SOX18 regulates the expression of matrix metalloproteinase 7 and guidance molecules in human endothelial cells.

PloS one

Hoeth M,Niederleithner H,Hofer-Warbinek R,Bilban M,Mayer H,Resch U,Lemberger C,Wagner O,Hofer E,Petzelbauer P,de Martin R

PA1-24474 was used in ChIP assay to study the target genes of transcription factor SOX18 in human endothelial cells

Mon Jun 04 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: SRY (sex determining region Y)-box 18; SRY box 18; Transcription factor SOX-18

Gene Aliases: HLTRS; HLTS; SOX18

UniProt ID: (Human) P35713

Entrez Gene ID: (Human) 54345

Molecular Function: HMG box transcription factor

negative regulation of transcription from RNA polymerase II promoter angiogenesis vasculogenesis in utero embryonic development hair follicle development vasculature development lymphangiogenesis heart looping outflow tract morphogenesis hair cycle process embryonic heart tube development mRNA transcription from RNA polymerase II promoter blood vessel endothelial cell migration negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell maturation stem cell fate specification endocardium formation lymphatic endothelial cell differentiation endocardial cell differentiation establishment of endothelial barrier regulation of stem cell proliferation

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription regulatory region DNA binding protein heterodimerization activity