Immunofluorescence analysis of SOX2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with Methanol for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SOX2 Rabbit Polyclonal Antibody (481400) at 2 ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Cytoskeleton was stained with alpha-Tubulin Monoclonal Antibody (Product# 322500, 1 ug/ml) followed by Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor® 594 conjugate (Product # A11032, 1:400). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Fruit fly, Human, Mouse, Chicken, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the N-terminal region of the human SOX2 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2-3 µg/ml|
|Immunofluorescence (IF)||2-3 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||5 ug|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The miR-20-Rest-Wnt signaling axis regulates neural progenitor cell differentiation.
48-1400 was used in immunocytochemistry to analyze regulation of neural progenitor cell differentiation by the miR-20-Rest-Wnt signaling axis
|Cui Y,Han J,Xiao Z,Chen T,Wang B,Chen B,Liu S,Han S,Fang Y,Wei J,Wang X,Ma X,Dai J||Scientific reports (6:null)||2016|
MiR-125b orchestrates cell proliferation, differentiation and migration in neural stem/progenitor cells by targeting Nestin.
48-1400 was used in immunocytochemistry to investigate the role of miR-125b in neural stem/progenitor cells
|Cui Y,Xiao Z,Han J,Sun J,Ding W,Zhao Y,Chen B,Li X,Dai J||BMC neuroscience (13:null)||2012|
A Branching Process to Characterize the Dynamics of Stem Cell Differentiation.
48-1400 was used in immunohistochemistry to create a mathematical model that predicts the average rates of proliferative and differentiative divisions in stem cell population
|Míguez DG||Scientific reports (5:null)||2015|
Sorting live stem cells based on Sox2 mRNA expression.
48-1400 was used in immunocytochemistry to report how stem cells can be purified using molecular beacons.
|Larsson HM,Lee ST,Roccio M,Velluto D,Lutolf MP,Frey P,Hubbell JA||PloS one (7:null)||2012|
Genome-wide analysis reveals that Smad3 and JMJD3 HDM co-activate the neural developmental program.
48-1400 was used in immunohistochemistry to report that neurogenesis is promoted by an interplay between the TGFβ pathway and JMJD3.
|Estarás C,Akizu N,García A,Beltrán S,de la Cruz X,Martínez-Balbás MA||Development (Cambridge, England) (139:2681)||2012|
Novel developmental biology-based protocol of embryonic stem cell differentiation to morphologically sound and functional yet immature hepatocytes.
48-1400 was used in flow cytometry to describe a method to generate mature hepatocytes from embryonic stem cells.
|Bukong TN,Lo T,Szabo G,Dolganiuc A||Liver international : official journal of the International Association for the Study of the Liver (32:732)||2012|
Sustained Wnt/ß-catenin signalling causes neuroepithelial aberrations through the accumulation of aPKC at the apical pole.
48-1400 was used in immunohistochemistry to report that beta-catenin controls the cell fate and polarity of neuroblasts by modulating the expression and localization of aPKC.
|Herrera A,Saade M,Menendez A,Marti E,Pons S||Nature communications (5:null)||2014|
|Mouse||Not Cited||SOX2 is a dose-dependent regulator of retinal neural progenitor competence.||Taranova OV,Magness ST,Fagan BM,Wu Y,Surzenko N,Hutton SR,Pevny LH||Genes and development (20:1187)||2006|
|Chicken||Not Cited||Vertebrate neurogenesis is counteracted by Sox1-3 activity.||Bylund M,Andersson E,Novitch BG,Muhr J||Nature neuroscience (6:1162)||2003|
|Human||Not Cited||Mutations in SOX2 cause anophthalmia.||Fantes J,Ragge NK,Lynch SA,McGill NI,Collin JR,Howard-Peebles PN,Hayward C,Vivian AJ,Williamson K,van Heyningen V,FitzPatrick DR||Nature genetics (33:461)||2003|
|Human||Not Cited||Cellular characterization of human pluripotent stem cells.||Quintanilla RH||Methods in molecular biology (Clifton, N.J.) (997:179)||2013|
|Fruit fly||Not Cited||The HMG-box transcription factor SoxNeuro acts with Tcf to control Wg/Wnt signaling activity.||Chao AT,Jones WM,Bejsovec A||Development (Cambridge, England) (134:989)||2007|