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Immunofluorescence analysis of SOX2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with Methanol for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SOX2 Rabbit Polyclonal Antibody (481400) at 2 ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Cytoskeleton was stained with alpha-Tubulin Monoclonal Antibody (Product# 322500, 1 ug/ml) followed by Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor® 594 conjugate (Product # A11032, 1:400). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). Panel d represents the merged image showing nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Fruit fly, Human, Mouse, Chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the N-terminal region of the human SOX2 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2-3 µg/ml|
|Immunofluorescence (IF)||2-3 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||5 ug|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Sustained Wnt/β-catenin signalling causes neuroepithelial aberrations through the accumulation of aPKC at the apical pole.
48-1400 was used in immunohistochemistry to report that beta-catenin controls the cell fate and polarity of neuroblasts by modulating the expression and localization of aPKC.
|Herrera A,Saade M,Menendez A,Marti E,Pons S||Nature communications (5:null)||2014|
|Mouse||Not Cited||SOX2 is a dose-dependent regulator of retinal neural progenitor competence.||Taranova OV,Magness ST,Fagan BM,Wu Y,Surzenko N,Hutton SR,Pevny LH||Genes & development (20:1187)||2006|
|Chicken||Not Cited||Vertebrate neurogenesis is counteracted by Sox1-3 activity.||Bylund M,Andersson E,Novitch BG,Muhr J||Nature neuroscience (6:1162)||2003|
|Human||Not Cited||Mutations in SOX2 cause anophthalmia.||Fantes J,Ragge NK,Lynch SA,McGill NI,Collin JR,Howard-Peebles PN,Hayward C,Vivian AJ,Williamson K,van Heyningen V,FitzPatrick DR||Nature genetics (33:461)||2003|
|Human||Not Cited||Cellular characterization of human pluripotent stem cells.||Quintanilla RH||Methods in molecular biology (Clifton, N.J.) (997:179)||2013|
|Fruit fly||Not Cited||The HMG-box transcription factor SoxNeuro acts with Tcf to control Wg/Wnt signaling activity.||Chao AT,Jones WM,Bejsovec A||Development (Cambridge, England) (134:989)||2007|
ANOP3; MCOPS3; sex determining region Y box 2; sex determining region Y-box 2; SRY (sex determining region Y)-box 2; SRY box 2; SRY-box containing gene 2; SRY-related HMG-box gene 2; transcription factor SOX-2; transcription factor SOX2
ANOP3; lcc; MCOPS3; Sox-2; SOX2; ysb