Proteintech

SPARC Polyclonal Antibody

SPARC Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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SPARC Antibody (15274-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using (Product # 15274-1-AP) (SPARC antibody) at dilution of 1:2,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Product Details

15274-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 25 publications 25 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

View 6 publications 6 publications

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Published species

Deer, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SPARC Fusion Protein Ag7390 (1-303 aa encoded by BC004974)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MRAWIFFLL CLAGRALAAP QQEALPDETE VVEETVAEVT EVSVGANPVQ VEVGEFDDGA EETEEEVVAE NPCQNHHCKH GKVCELDENN TPMCVCQDPT SCPAPIGEFE KVCSNDNKTF DSSCHFFATK CTLEGTKKGH KLHLDYIGPC KYIPPCLDSE LTEFPLRMRD WLKNVLVTLY ERDEDNNLLT EKQKLRVKKI HENEKRLEAG DHPVELLARD FEKNYNMYIF PVHWQFGQLD QHPIDGYLSH TELAPLRAPL IPMEHCTTRF FETCDLDNDK YIALDEWAGC FGIKQKDIDK DLVI (1-303 aa encoded by BC004974)

Target Information

SPARC gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

SPARC in cancer-associated fibroblasts is an independent poor prognostic factor in non-metastatic triple-negative breast cancer and exhibits pro-tumor activity.

International journal of cancer

Alcaraz LB,Mallavialle A,Mollevi C,Boissière-Michot F,Mansouri H,Simony-Lafontaine J,Laurent-Matha V,Chardès T,Jacot W,Turtoi A,Roger P,Guiu S,Liaudet-Coopman E

Wed Mar 15 00:00:00 UTC 2023

Tissue-resident CXCR4⁺ macrophage as a poor prognosis signature promotes pancreatic ductal adenocarcinoma progression.

International journal of cancer

Liao Z,Ye L,Li T,Jin X,Lin X,Fei Q,Zhang H,Shi S,Yu X,Jin K,Wu W

Thu Jun 01 00:00:00 UTC 2023

SPARC expression in tumor microenvironment induces partial epithelial-to-mesenchymal transition of esophageal adenocarcinoma cells via cooperating with TGF-β signaling.

Cell biology international

Ma W,Yan Y,Bai S,Zhou Y,Wang X,Feng Z,Li G,Zhou S,Zhang J,Ren J

Sun Jan 01 00:00:00 UTC 2023

SPARC expression in tumor microenvironment induces partial epithelial-to-mesenchymal transition of esophageal adenocarcinoma cells via cooperating with TGF-β signaling.

Cell biology international

Ma W,Yan Y,Bai S,Zhou Y,Wang X,Feng Z,Li G,Zhou S,Zhang J,Ren J

Sun Jan 01 00:00:00 UTC 2023

Atorvastatin reduces IOP in ocular hypertension in vivo and suppresses ECM in trabecular meshwork perhaps via FGD4.

International journal of molecular medicine

Song XY,Chen YY,Liu WT,Cong L,Zhang JL,Zhang Y,Zhang YY

Wed Jun 01 00:00:00 UTC 2022

Atorvastatin reduces IOP in ocular hypertension in vivo and suppresses ECM in trabecular meshwork perhaps via FGD4.

International journal of molecular medicine

Song XY,Chen YY,Liu WT,Cong L,Zhang JL,Zhang Y,Zhang YY

Wed Jun 01 00:00:00 UTC 2022

SARS-CoV-2 Disrupts Proximal Elements in the JAK-STAT Pathway.

Journal of virology

Chen DY,Khan N,Close BJ,Goel RK,Blum B,Tavares AH,Kenney D,Conway HL,Ewoldt JK,Chitalia VC,Crossland NA,Chen CS,Kotton DN,Baker SC,Fuchs SY,Connor JH,Douam F,Emili A,Saeed M

Thu Sep 09 00:00:00 UTC 2021

A 9-kDa matricellular SPARC fragment released by cathepsin D exhibits pro-tumor activity in the triple-negative breast cancer microenvironment.

Theranostics

Alcaraz LB,Mallavialle A,David T,Derocq D,Delolme F,Dieryckx C,Mollevi C,Boissière-Michot F,Simony-Lafontaine J,Du Manoir S,Huesgen PF,Overall CM,Tartare-Deckert S,Jacot W,Chardès T,Guiu S,Roger P,Reinheckel T,Moali C,Liaudet-Coopman E

Wed Jul 28 00:00:00 UTC 2021

A 9-kDa matricellular SPARC fragment released by cathepsin D exhibits pro-tumor activity in the triple-negative breast cancer microenvironment.

Theranostics

Wed Jul 28 00:00:00 UTC 2021

SEC23A Inhibit Melanoma Metastatic through Secretory PF4 Cooperation with SPARC to Inhibit MAPK Signaling Pathway.

International journal of biological sciences

Zeng B,Sun Z,Zhao Q,Liu D,Chen H,Li X,Xing HR,Wang J

Fri Mar 18 00:00:00 UTC 2022

The "Matrisome" reveals the characterization of skin keloid microenvironment.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang S,Liu B,Wang W,Lv L,Gao D,Chai M,Li M,Wu Z,Zhu Y,Ma J,Leng L

Thu Apr 01 00:00:00 UTC 2021

The "Matrisome" reveals the characterization of skin keloid microenvironment.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang S,Liu B,Wang W,Lv L,Gao D,Chai M,Li M,Wu Z,Zhu Y,Ma J,Leng L

Thu Apr 01 00:00:00 UTC 2021

The Balance between Orthodontic Force and Radiation in the Jawbone: Microstructural, Histological, and Molecular Study in a Rat Model.

Biology

Dorchin-Ashkenazi H,Ginat-Koton R,Gabet Y,Klein Y,Chaushu S,Dorchin H,Brosh T,Vered M

Thu Nov 18 00:00:00 UTC 2021

MicroRNA‑592 promotes cell proliferation, migration and invasion in colorectal cancer by directly targeting SPARC.

Molecular medicine reports

Pan Z,Xie R,Song W,Gao C

Thu Apr 01 00:00:00 UTC 2021

Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

International journal of molecular sciences

Morani F,Bisceglia L,Rosini G,Mutti L,Melaiu O,Landi S,Gemignani F

Mon Mar 08 00:00:00 UTC 2021

Evidence for Delivery of Abraxane via a Denatured-Albumin Transport System.

ACS applied materials & interfaces

Hama M,Ishima Y,Chuang VTG,Ando H,Shimizu T,Ishida T

Wed May 05 00:00:00 UTC 2021

SPARC knockdown attenuated TGF-β1-induced fibrotic effects through Smad2/3 pathways in human pterygium fibroblasts.

Archives of biochemistry and biophysics

Fan J,Zhang X,Jiang Y,Chen L,Sheng M,Chen Y

Tue Nov 30 00:00:00 UTC 2021

Effects of transforming growth factor-β1 on plasminogen activation in stem cells from the apical papilla: role of activating receptor-like kinase 5/Smad2 and mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) signalling.

International endodontic journal

Chang MC,Chang HH,Hsieh WC,Huang WL,Lian YC,Jeng PY,Wang YL,Yeung SY,Jeng JH

Fri May 01 00:00:00 UTC 2020

SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway.

bioRxiv : the preprint server for biology

Chen DY,Khan N,Close BJ,Goel RK,Blum B,Tavares AH,Kenney D,Conway HL,Ewoldt JK,Kapell S,Chitalia VC,Crossland NA,Chen CS,Kotton DN,Baker SC,Connor JH,Douam F,Emili A,Saeed M

Wed Oct 28 00:00:00 UTC 2020

Induction of SPARC on Oxidative Stress, Inflammatory Phenotype Transformation, and Apoptosis of Human Brain Smooth Muscle Cells Via TGF-β1-NOX4 Pathway.

Journal of molecular neuroscience : MN

Tan X,Li T,Zhu S,Zhong W,Li F,Wang Y

Sun Nov 01 00:00:00 UTC 2020

Priming integrin α5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix-receptor activity.

Journal of cellular physiology

Wang H,Ning T,Song C,Luo X,Xu S,Zhang X,Deng Z,Ma D,Wu B

Thu Aug 01 00:00:00 UTC 2019

Priming integrin α5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix-receptor activity.

Journal of cellular physiology

Wang H,Ning T,Song C,Luo X,Xu S,Zhang X,Deng Z,Ma D,Wu B

Thu Aug 01 00:00:00 UTC 2019

Priming integrin α5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix-receptor activity.

Journal of cellular physiology

Wang H,Ning T,Song C,Luo X,Xu S,Zhang X,Deng Z,Ma D,Wu B

Thu Aug 01 00:00:00 UTC 2019

Priming integrin α5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix-receptor activity.

Journal of cellular physiology

Wang H,Ning T,Song C,Luo X,Xu S,Zhang X,Deng Z,Ma D,Wu B

Thu Aug 01 00:00:00 UTC 2019

Myosin-X Silencing in the Trabecular Meshwork Suggests a Role for Tunneling Nanotubes in Outflow Regulation.

Investigative ophthalmology & visual science

Sun YY,Yang YF,Keller KE

Fri Feb 01 00:00:00 UTC 2019

Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration.

Journal of proteomics

Dong Z,Coates D,Liu Q,Sun H,Li C

Wed Mar 20 00:00:00 UTC 2019

Quantitative proteomic analysis of deer antler stem cells as a model of mammalian organ regeneration.

Journal of proteomics

Dong Z,Coates D,Liu Q,Sun H,Li C

Wed Mar 20 00:00:00 UTC 2019

Olfactory ensheathing cells facilitate neurite sprouting and outgrowth by secreting high levels of hevin.

Journal of chemical neuroanatomy

Ge L,Zhuo Y,Wu P,Liu Y,Qi L,Teng X,Duan D,Chen P,Lu M

Tue Nov 26 00:00:00 UTC 2019

Antimetastatic Effect of Fucoidan-Sargassum against Liver Cancer Cell Invadopodia Formation via Targeting Integrin αVβ3 and Mediating αVβ3/Src/E2F1 Signaling.

Journal of Cancer

Pan TJ,Li LX,Zhang JW,Yang ZS,Shi DM,Yang YK,Wu WZ

Wed Sep 30 00:00:00 UTC 2020

Transcriptome analysis of gingival tissues of enamel-renal syndrome.

Journal of periodontal research

Wang YP,Lin HY,Zhong WL,Simmer JP,Wang SK

Sun Dec 01 00:00:00 UTC 2019

Effects of hydrogen peroxide on biological characteristics and osteoinductivity of decellularized and demineralized bone matrices.

Journal of biomedical materials research. Part A

Qing Q,Zhang YJ,Yang JL,Ning LJ,Zhang YJ,Jiang YL,Zhang Y,Luo JC,Qin TW

Mon Jul 01 00:00:00 UTC 2019

StarD13 3'-untranslated region functions as a ceRNA for TP53INP1 in prohibiting migration and invasion of breast cancer cells by regulating miR-125b activity.

European journal of cell biology

Zheng L,Li X,Chou J,Xiang C,Guo Q,Zhang Z,Guo X,Gao L,Xing Y,Xi T

Mon Jan 01 00:00:00 UTC 2018

StarD13 3'-untranslated region functions as a ceRNA for TP53INP1 in prohibiting migration and invasion of breast cancer cells by regulating miR-125b activity.

European journal of cell biology

Zheng L,Li X,Chou J,Xiang C,Guo Q,Zhang Z,Guo X,Gao L,Xing Y,Xi T

Mon Jan 01 00:00:00 UTC 2018

Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.

Proteome science

Darville LNF,Sokolowski BHA

Fri Mar 18 00:00:00 UTC 2022

Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.

Proteome science

Darville LNF,Sokolowski BHA

Fri Mar 18 00:00:00 UTC 2022

Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.

Proteome science

Darville LNF,Sokolowski BHA

Fri Mar 18 00:00:00 UTC 2022

Label-free quantitative mass spectrometry analysis of differential protein expression in the developing cochlear sensory epithelium.

Proteome science

Darville LNF,Sokolowski BHA

Fri Mar 18 00:00:00 UTC 2022

Proteomic Analysis of Plasma Membrane Proteins of Antler Stem Cells Using Label-Free LC⁻MS/MS.

International journal of molecular sciences

Wang D,Ba H,Li C,Zhao Q,Li C

Mon Nov 05 00:00:00 UTC 2018

Proteomic Analysis of Plasma Membrane Proteins of Antler Stem Cells Using Label-Free LC⁻MS/MS.

International journal of molecular sciences

Wang D,Ba H,Li C,Zhao Q,Li C

Mon Nov 05 00:00:00 UTC 2018

miR-504 promotes tumour growth and metastasis in human osteosarcoma by targeting TP53INP1.

Oncology reports

Cai Q,Zeng S,Dai X,Wu J,Ma W

Wed Nov 01 00:00:00 UTC 2017

Analysis of colorectal cancer glyco-secretome identifies laminin β-1 (LAMB1) as a potential serological biomarker for colorectal cancer.

Proteomics

Lin Q,Lim HS,Lin HL,Tan HT,Lim TK,Cheong WK,Cheah PY,Tang CL,Chow PK,Chung MC

Sun Nov 01 00:00:00 UTC 2015

Analysis of colorectal cancer glyco-secretome identifies laminin β-1 (LAMB1) as a potential serological biomarker for colorectal cancer.

Proteomics

Lin Q,Lim HS,Lin HL,Tan HT,Lim TK,Cheong WK,Cheah PY,Tang CL,Chow PK,Chung MC

Sun Nov 01 00:00:00 UTC 2015

Mapping molecular differences and extracellular matrix gene expression in segmental outflow pathways of the human ocular trabecular meshwork.

PloS one

Vranka JA,Bradley JM,Yang YF,Keller KE,Acott TS

Mon Mar 21 00:00:00 UTC 2016

Mapping molecular differences and extracellular matrix gene expression in segmental outflow pathways of the human ocular trabecular meshwork.

PloS one

Vranka JA,Bradley JM,Yang YF,Keller KE,Acott TS

Mon Mar 21 00:00:00 UTC 2016

Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation.

Journal of proteomics

Chen SX,Xu XE,Wang XQ,Cui SJ,Xu LL,Jiang YH,Zhang Y,Yan HB,Zhang Q,Qiao J,Yang PY,Liu F

Tue Oct 14 00:00:00 UTC 2014

Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation.

Journal of proteomics

Chen SX,Xu XE,Wang XQ,Cui SJ,Xu LL,Jiang YH,Zhang Y,Yan HB,Zhang Q,Qiao J,Yang PY,Liu F

Tue Oct 14 00:00:00 UTC 2014

Expression of Secreted Protein Acidic and Rich in Cysteine in the Stroma of a Colorectal Carcinoma is Associated With Patient Prognosis.

Annals of coloproctology

Kim JY,Jeong D,Ahn TS,Kim HJ,Park DS,Park SY,Bae SB,Lee S,Lee SS,Lee MS,Cho HD,Baek MJ

Sat Jun 01 00:00:00 UTC 2013

Expression of Secreted Protein Acidic and Rich in Cysteine in the Stroma of a Colorectal Carcinoma is Associated With Patient Prognosis.

Annals of coloproctology

Kim JY,Jeong D,Ahn TS,Kim HJ,Park DS,Park SY,Bae SB,Lee S,Lee SS,Lee MS,Cho HD,Baek MJ

Sat Jun 01 00:00:00 UTC 2013

nab-Paclitaxel potentiates gemcitabine activity by reducing cytidine deaminase levels in a mouse model of pancreatic cancer.

Cancer discovery

Frese KK,Neesse A,Cook N,Bapiro TE,Lolkema MP,Jodrell DI,Tuveson DA

Thu Mar 01 00:00:00 UTC 2012

nab-Paclitaxel potentiates gemcitabine activity by reducing cytidine deaminase levels in a mouse model of pancreatic cancer.

Cancer discovery

Frese KK,Neesse A,Cook N,Bapiro TE,Lolkema MP,Jodrell DI,Tuveson DA

Thu Mar 01 00:00:00 UTC 2012

The pancreas cancer microenvironment.

Clinical cancer research : an official journal of the American Association for Cancer Research

Feig C,Gopinathan A,Neesse A,Chan DS,Cook N,Tuveson DA

Wed Aug 15 00:00:00 UTC 2012

The pancreas cancer microenvironment.

Clinical cancer research : an official journal of the American Association for Cancer Research

Feig C,Gopinathan A,Neesse A,Chan DS,Cook N,Tuveson DA

Wed Aug 15 00:00:00 UTC 2012

Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer.

Journal of proteome research

Loei H,Tan HT,Lim TK,Lim KH,So JB,Yeoh KG,Chung MC

Fri Mar 02 00:00:00 UTC 2012

Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer.

Journal of proteome research

Loei H,Tan HT,Lim TK,Lim KH,So JB,Yeoh KG,Chung MC

Fri Mar 02 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: Basement membrane protein 40; Basement-membrane protein 40; BM-40; BM40; Cysteine rich protein; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted acidic cystein-rich glycoprotein (osteonectin); secreted acidic cysteine rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic and rich in cysteine (SPARC); secreted protein, acidic, cysteine-rich (osteonectin); SPARC

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Gene Aliases: BM-40; OI17; ON; SPARC

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UniProt ID: (Human) P09486, (Pig) P20112, (Rat) P16975, (Mouse) P07214

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Entrez Gene ID: (Human) 6678, (Pig) 595124, (Rat) 24791, (Mouse) 20692

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Function(s)

calcium ion binding protein binding collagen binding extracellular matrix binding metal ion binding extracellular matrix glycoprotein

Process(es)

ossification negative regulation of endothelial cell proliferation platelet degranulation receptor-mediated endocytosis signal transduction heart development response to gravity response to lead ion positive regulation of endothelial cell migration negative regulation of angiogenesis regulation of cell morphogenesis extracellular matrix organization lung development response to lipopolysaccharide response to L-ascorbic acid response to cytokine wound healing response to peptide hormone response to ethanol response to cadmium ion inner ear development response to glucocorticoid response to cAMP response to calcium ion bone development cellular response to growth factor stimulus regulation of cell proliferation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SPARC Polyclonal Antibody

SPARC Antibody (15274-1-AP) in IHC (P)

Product Details

15274-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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