Search
Bioss
SPARC Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 7
SPARC Antibody (BS-1133R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with SPARC Polyclonal Antibody, Unconjugated (bs-1133R) at 1:500 overnight at 4°C, followed by a conjugated secondary Goat Anti-Rabbit IgG, C... View More
Product Details
BS-1133R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human SPARC, amino acids 101-200.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
SPARC gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Basement membrane protein 40; Basement-membrane protein 40; BM-40; BM40; Cysteine rich protein; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted acidic cystein-rich glycoprotein (osteonectin); secreted acidic cysteine rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic and rich in cysteine (SPARC); secreted protein, acidic, cysteine-rich (osteonectin); SPARC; unnamed protein product
Gene Aliases: BM-40; OI17; ON; ONT; SPARC
UniProt ID: (Human) P09486, (Rat) P16975, (Mouse) P07214
Entrez Gene ID: (Human) 6678, (Rat) 24791, (Mouse) 20692
negative regulation of endothelial cell proliferation
positive regulation of endothelial cell migration
negative regulation of angiogenesis
regulation of cell morphogenesis
semicircular canal morphogenesis
regulation of synapse organization
ossification
signal transduction
heart development
response to gravity
response to lead ion
lung development
response to lipopolysaccharide
response to L-ascorbic acid
response to cytokine
wound healing
regulation of cell proliferation
response to peptide hormone
response to ethanol
response to cadmium ion
inner ear development
response to glucocorticoid
response to cAMP
response to calcium ion
bone development
cellular response to growth factor stimulus
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support