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Invitrogen
SPDYA Polyclonal Antibody
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FIGURE: 1 / 1
SPDYA Antibody (PA5-96075) in WB
Western blot analysis of SPDYA in extracts of various cell lines. Samples were incubated with SPDYA Polyclonal antibody (Product # PA5-96075) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.
Product Details
PA5-96075
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human SPDYA (NP_8774332)
Conjugate
Form
Liquid
Concentration
0.14 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MRHNQMCCET PPTVTVYVKS GSNRSHQPKK PITLKRPICK DNWQAFEKNT HNNNKSKRPK GPCLVIQRQD MTAFFKLFDD DLIQDFLWMD CCCKIADKYL LAMTFVYFKR AKFTISEHTR INFFIALYLA NTVEEDEEET KYEIFPWALG KNWRKLFPNF LKLRDQLWDR IDYRAIVSRR CCEEVMAIAP THYIWQRERS VHHSGAVRNY NRDEVQLPRG PSATPVDCSL CGKKRRYVRL GLSSSSSLSS HTAGVTEKHS QDSYNSLSMD IIGDPSQAYT GSEVVNDHQS NKGKKTNFLK KDKSMEWFTG SEE; Positive Samples: A-549, Mouse ovary, Rat testis; Cellular Location: Nucleus
Target Information
Speedy A, also known as SPDYA, SPDY1, Ringo3 or SPY1, is a 313 amino acid protein that localizes to the nucleus and belongs to the speedy/ringo family. Expressed at high levels in testis and at lower levels in brain, kidney, heart, bone marrow, colon, lung, liver spleen and placenta, Speedy A functions to regulate the G1/S phase transition of the cell cycle, specifically by binding to and activating Cdc2 p34, Cdk2 and p27. Additionally, Speedy A mediates cell survival during DNA damage, suggesting that Speedy A plays a role in proper cell cycle progression throughout the life of the cell. Multiple isoforms of Speedy A exist due to alternative splicing events. The gene encoding Speedy A maps to human chromosome 2, which encodes over 1,400 genes and comprises nearly 8% of the human genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GS4; hSpy/Ringo A; MLZ-465; mSpy/Ringo A; Protein expressed in male leptotene and zygotene spermatocytes 465; Rapid inducer of G2/M progression in oocytes A; RINGO A; SPDYA; spdya {ECO:0000250|UniProtKB:Q5MJ70}; speedy A; speedy A1; speedy A2; speedy homolog 1; speedy homolog A; Speedy protein A; Speedy-1; speedy/ringo; spermatogenesis related protein Gs4; Spy1
Gene Aliases: 4921517J08Rik; 4930548B21Rik; GS4; Lm23; MLZ-465; RINGO3; RINGOA; SPDY1; SPDYA; SPY1
UniProt ID: (Human) Q5MJ70, (Mouse) Q5IBH7, (Rat) Q8R496
Entrez Gene ID: (Human) 245711, (Mouse) 70891, (Rat) 192209
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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