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SPOP Antibody (PA5-28522) in WB
Western Blot analysis of SPOP was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a SPOP Polyclonal Antibody (Product # PA5-28522) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Product Details
PA5-28522
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 223 of Human SPOP
Conjugate
Unconjugated
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2545998
Product Specific Information
Recommended positive controls: HepG2, HepG2 nuclear extract, Mouse brain, SPOP-transfected 293T.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (99%), Xenopus laevis (100%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HIB homolog 1; PDX-1 C-terminal-interacting factor 1; Roadkill homolog 1; speckle type POZ protein; Speckle-type POZ protein
Gene Aliases: AI315626; BTBD32; Pcif1; SPOP; TEF2
UniProt ID: (Human) O43791, (Mouse) Q6ZWS8
Entrez Gene ID: (Human) 8405, (Mouse) 20747
regulation of proteolysis
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of transcription from RNA polymerase II promoter
glucose homeostasis
negative regulation of sequence-specific DNA binding transcription factor activity
positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of type B pancreatic cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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