Proteintech

SPR Polyclonal Antibody

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SPR Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using SPR antibody 16822-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

SPR Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 16822-1-AP ( SPR Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

SPR Antibody in Western Blot (WB) — Human liver tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SPR Antibody in Immunoprecipitation (IP) — IP result of anti-SPR (IP:16822-1-AP, 4ug; Detection:16822-1-AP 1:500) with mouse liver tissue lysate 4000ug.

Product Details

16822-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:400	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SPR Fusion Protein Ag10395 (1-261 aa encoded by BC017310) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.3 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MEGGLGRAV CLLTGASRGF GRTLAPLLAS LLSPGSVLVL SARNDEALRQ LEAELGAERS GLRVVRVPAD LGAEAGLQQL LGALRELPRP KGLQRLLLIN NAGSLGDVSK GFVDLSDSTQ VNNYWALNLT SMLCLTSSVL KAFPDSPGLN RTVVNISSLC ALQPFKGWAL YCAGKAARDM LFQVLALEEP NVRVLNYAPG PLDTDMQQLA RETSVDPDMR KGLQELKAKG KLVDCKVSAQ KLLSLLEKDE FKSGAHVDFY DK (1-261 aa encoded by BC017310)

Target Information

SPR encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.