Proteintech

SPR Polyclonal Antibody

View all (33) SPR antibodies

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SPR Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of Hela cells, using SPR antibody 16822-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

SPR Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 16822-1-AP ( SPR Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

SPR Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 16822-1-AP ( SPR Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

SPR Antibody in Western Blot (WB) — Human liver tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SPR Antibody in Western Blot (WB) — Rat liver tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

SPR Antibody in Western Blot (WB) — Rat heart tissue were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

SPR Antibody in Western Blot (WB) — K-562 cells were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SPR Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 16822-1-AP (SPR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SPR Antibody in Immunoprecipitation (IP) — IP result of anti-SPR (IP:16822-1-AP, 4ug; Detection:16822-1-AP 1:500) with mouse liver tissue lysate 4000ug.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:10-1:100

Immunofluorescence (IF)

1:10-1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SPR Fusion Protein Ag10395

Conjugate

Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

SPR encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: SDR38C1; Sepiapterin reductase; short chain dehydrogenase/reductase family 38C, member 1; SPR

Gene Aliases: AA409688; ENSMUSG00000071345; Gm10328; SDR38C1; SPR

UniProt ID: (Human) P35270, (Rat) P18297

Entrez Gene ID: (Human) 6697, (Mouse) 20751, (Rat) 29270

Molecular Function: dehydrogenase oxidoreductase reductase

tetrahydrobiopterin biosynthetic process nitric oxide biosynthetic process regulation of nitric-oxide synthase activity oxidation-reduction process L-phenylalanine metabolic process metabolic process response to organic substance obsolete death pteridine metabolic process regulation of multicellular organism growth norepinephrine metabolic process dopamine metabolic process serotonin metabolic process tetrahydrobiopterin metabolic process cell morphogenesis involved in neuron differentiation voluntary musculoskeletal movement

Process(es)

aldo-keto reductase (NADP) activity sepiapterin reductase activity NADP binding oxidoreductase activity protein homodimerization activity