Invitrogen

SPR Polyclonal Antibody

Product Image Gallery

SPR Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of SPR in methanol-fixed HeLa cells using a SPR polyclonal antibody (Product # PA5-22293) at a 1:200 dilution.

SPR Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — SPR Polyclonal Antibody detects SPR protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SPR Polyclonal Antibody (Product # PA5-22293) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

SPR Antibody in Western Blot (WB) — Western Blot using SPR Polyclonal Antibody (Product # PA5-22293). Sample (30 µg of whole cell lysate). Lane A: A431. 12% SDS PAGE. SPR Polyclonal Antibody (Product # PA5-22293) diluted at 1:1,000.

SPR Antibody in Immunoprecipitation (IP) — SPR Polyclonal Antibody immunoprecipitates SPR protein in IP experiments. IP samples: A431 whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of SPR protein by 4 µg SPR Polyclonal Antibody (Product # PA5-22293). 5 % SDS-PAGE. The immunoprecipitated SPR protein was detected by SPR Polyclonal Antibody (Product # PA5-22293) diluted at 1:500.

Product Details

PA5-22293

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunoprecipitation (IP)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 65 and 261 of SPR (Uniprot ID#P35270) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.1M tris glycine, pH 7, with 20% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11153773

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2.

Predicted reactivity: Rabbit (86%), Rhesus Monkey (97%), Bovine (82%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

SPR encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.