Invitrogen

SPR Polyclonal Antibody

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SPR Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of SPR in methanol-fixed HeLa cells using a SPR polyclonal antibody (Product # PA5-22293) at a 1:200 dilution.

SPR Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of SPR in the cytosol of paraffin-embedded mouse fore brain tissue using a SPR polyclonal antibody (Product # PA5-22293) at a dilution of 1:500.

SPR Antibody in Western Blot (WB) — Western blot analysis of SPR using 30 µg of A431 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a SPR polyclonal antibody (Product # PA5-22293) at a dilution of 1:1000.

SPR Antibody in Immunoprecipitation (IP) — PA5-22293 immunoprecipitates SPR protein in IP experiments. IP samples: A431 whole cell extract A. Control with 4 µg of preimmune Rabbit IgG B. Immunoprecipitation of SPR protein by 4 µg SPR antibody (Product # PA5-22293) on 5% SDS-PAGE.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunoprecipitation (IP)

Assay-Dependent

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 65 and 261 of SPR (Uniprot ID#P35270)

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11153773

Product Specific Information

PA5-22293 targets SPR in ICC, IF, and WB applications and shows reactivity with Human samples.

The PA5-22293 immunogen is a recombinant fragment corresponding to a region within amino acids 65 and 261 of SPR (Uniprot ID#P35270).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: member 1; SDR38C1; Sepiapterin reductase; short chain dehydrogenase/reductase family 38C; short chain dehydrogenase/reductase family 38C, member 1; SPR

Gene Aliases: AA409688; ENSMUSG00000071345; Gm10328; SDR38C1; SPR

UniProt ID: (Human) P35270

Entrez Gene ID: (Human) 6697, (Mouse) 20751

Molecular Function: dehydrogenase oxidoreductase reductase