Invitrogen

SPR Polyclonal Antibody

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SPR Antibody in Western Blot (WB) — Western blot analysis of SPR in Lane 1: human HepG2 whole cell lysate, Lane 2: mouse HEPA1-6 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with SPR polyclonal antibody (Product # PA5-80068) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000. Signal development was performed using a chemiluminescence (ECL) kit.

Product Details

Tested Applications

Dilution

Western Blot (WB)

0.1-0.5 µg/mL

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived human SPR recombinant protein (Position: V36-K261).

Conjugate

Unconjugated

Form

Lyophilized

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

RRID

AB_2747183

Product Specific Information

Add 0.2 mL of distilled water, will yield a concentration of 500 µg/mL.

Target Information

SPR encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Sepiapterin reductase; short chain dehydrogenase/reductase family 38C, member 1; SPR

Gene Aliases: AA409688; ENSMUSG00000071345; Gm10328; SDR38C1; SPR

UniProt ID: (Human) P35270

Entrez Gene ID: (Human) 6697, (Mouse) 20751

Molecular Function: dehydrogenase oxidoreductase reductase

tetrahydrobiopterin biosynthetic process nitric oxide biosynthetic process regulation of nitric-oxide synthase activity oxidation-reduction process L-phenylalanine metabolic process metabolic process response to organic substance obsolete death pteridine metabolic process regulation of multicellular organism growth norepinephrine metabolic process dopamine metabolic process serotonin metabolic process tetrahydrobiopterin metabolic process cell morphogenesis involved in neuron differentiation voluntary musculoskeletal movement

Process(es)

aldo-keto reductase (NADP) activity sepiapterin reductase activity NADP binding oxidoreductase activity protein homodimerization activity