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SPTBN1 Polyclonal Antibody

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Antibody Testing Data (10)

SPTBN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 10

SPTBN1 Antibody (19722-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using (Product # 19722-1-AP) (SPTBN1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Product Image Gallery

SPTBN1 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with (Product # 19722-1-AP) SPTBN1-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

SPTBN1 Antibody in Immunoprecipitation (IP) — IP result of anti-SPTBN1-Specific (IP:19722-1-AP, 5ug; Detection:19722-1-AP 1:600) with HEK-293 cells lysate 1000ug.

Product Details

19722-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

Spectrin (Sp), the most abundant of the erythrocyte membrane skeleton proteins, helps these cells maintain their characteristic biconcave shape while remaining flexible and elastic. Erythrocyte Sp is a heterodimer composed of a 280 kDa alpha subunit and a 246 kDa beta subunit which associate in a side-to-side, antiparallel configuration to form a 100 nm rod-like structure. Sp in other tissues may be composed of distinct but homologous alpha and beta subunits, sometimes referred to as fodrin. A newly introduced nomenclature designates the Sp subunits of the erythrocyte as alpha-1 and beta-1, and the fodrin subunits as alpha-2 and beta-2. Alternatively spliced forms of each are designated as epsilon-1, epsilon-2, etc. (e. g. beta-1 epsilon-1).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Analytical chemistry

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MiR-362 suppresses cervical cancer progression via directly targeting BAP31 and activating TGFβ/Smad pathway.

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Yang S,Sun Y,Jiang D,Wang J,Dang E,Li Z,Zhou J,Lu Y,Shi J,Tao L,Wang J,Jin B,Zheng L,Yang K

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USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Nature communications

Li X,Song N,Liu L,Liu X,Ding X,Song X,Yang S,Shan L,Zhou X,Su D,Wang Y,Zhang Q,Cao C,Ma S,Yu N,Yang F,Wang Y,Yao Z,Shang Y,Shi L

Fri Mar 31 00:00:00 EDT 2017

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Nature communications

Li X,Song N,Liu L,Liu X,Ding X,Song X,Yang S,Shan L,Zhou X,Su D,Wang Y,Zhang Q,Cao C,Ma S,Yu N,Yang F,Wang Y,Yao Z,Shang Y,Shi L

Fri Mar 31 00:00:00 EDT 2017

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Nature communications

Li X,Song N,Liu L,Liu X,Ding X,Song X,Yang S,Shan L,Zhou X,Su D,Wang Y,Zhang Q,Cao C,Ma S,Yu N,Yang F,Wang Y,Yao Z,Shang Y,Shi L

Fri Mar 31 00:00:00 EDT 2017

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Nature communications

Li X,Song N,Liu L,Liu X,Ding X,Song X,Yang S,Shan L,Zhou X,Su D,Wang Y,Zhang Q,Cao C,Ma S,Yu N,Yang F,Wang Y,Yao Z,Shang Y,Shi L

Fri Mar 31 00:00:00 EDT 2017

Identification of nuclear phosphoproteins as novel tobacco markers in mouse lung tissue following short-term exposure to tobacco smoke.

FEBS open bio

Niimori-Kita K,Ogino K,Mikami S,Kudoh S,Koizumi D,Kudoh N,Nakamura F,Misumi M,Shimomura T,Hasegawa K,Usui F,Nagahara N,Ito T

Tue Oct 28 00:00:00 EDT 2014

Identification of nuclear phosphoproteins as novel tobacco markers in mouse lung tissue following short-term exposure to tobacco smoke.

FEBS open bio

Niimori-Kita K,Ogino K,Mikami S,Kudoh S,Koizumi D,Kudoh N,Nakamura F,Misumi M,Shimomura T,Hasegawa K,Usui F,Nagahara N,Ito T

Tue Oct 28 00:00:00 EDT 2014

Global profiling of proteolytically modified proteins in human metastatic hepatocellular carcinoma cell lines reveals CAPN2 centered network.

Proteomics

Shen C,Yu Y,Li H,Yan G,Liu M,Shen H,Yang P

Fri Jun 01 00:00:00 EDT 2012

Global profiling of proteolytically modified proteins in human metastatic hepatocellular carcinoma cell lines reveals CAPN2 centered network.

Proteomics

Shen C,Yu Y,Li H,Yan G,Liu M,Shen H,Yang P

Fri Jun 01 00:00:00 EDT 2012

Global profiling of proteolytically modified proteins in human metastatic hepatocellular carcinoma cell lines reveals CAPN2 centered network.

Proteomics

Shen C,Yu Y,Li H,Yan G,Liu M,Shen H,Yang P

Fri Jun 01 00:00:00 EDT 2012

Global profiling of proteolytically modified proteins in human metastatic hepatocellular carcinoma cell lines reveals CAPN2 centered network.

Proteomics

Shen C,Yu Y,Li H,Yan G,Liu M,Shen H,Yang P

Fri Jun 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: beta fodrin; beta-fodrin; beta-G spectrin; Beta-II spectrin; beta-spectrin 2; beta-spectrin 2, non-erythrocytic; beta-spectrin II; beta-spectrin non-erythrocytic 1; brain spectrin; embryonic liver beta-fodrin; Embryonic liver fodrin; embryonic liver fodrin beta chain; epididymis luminal protein 102; Fodrin beta chain; non-erythrocytic; spectrin beta 2; spectrin beta chain, brain 1; Spectrin beta chain, non-erythrocytic 1; spectrin G; spectrin, beta, non-erythrocytic 1; Spectrin, non-erythroid beta chain 1

Gene Aliases: 9930031C03Rik; AL033301; betaSpII; ELF; elf1; elf3; HEL102; mKIAA4049; Spnb-2; Spnb2; SPTB2; SPTBN1

UniProt ID: (Human) Q01082, (Mouse) Q62261

Entrez Gene ID: (Human) 6711, (Mouse) 20742

Function(s)