Invitrogen

SPTBN1 Polyclonal Antibody

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Datasheet

Protocols

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SPTBN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of paraffin-embedded human liver carcinoma tissue using SPTBN1 Polyclonal Antibody (Product # PA5-119749). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the SPTBN1 antibody at a dilution of 1:800 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

SPTBN1 Antibody in Western Blot (WB) — Western blot analysis of SPTBN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. SPTBN1 Polyclonal Antibody (Product # PA5-119749) at 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW1990 cell lysate. Lane 2: PANC-1 cell lysate. Lane 3: Rat brain tissue lysate. Lane 4: Mouse brain tissue lysate. Lane 5: Mouse cerebellum tissue lysate.

Product Details

PA5-119749

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide within human sptbn1 aa 2320-2364

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2913321

Product Specific Information

Positive Control: SW1990 cell lysate, PANC-1 cell lysate, rat brain tissue lysate, mouse brain tissue lysate, mouse cerebellum tissue lysate, rat kidney tissue, human liver carcinoma tissue, mouse brain tissue.

Subcellular Location: Cell membrane, Cytoplasm, Cytoskeleton, Membrane.

Target Information

Spectrin (Sp), the most abundant of the erythrocyte membrane skeleton proteins, helps these cells maintain their characteristic biconcave shape while remaining flexible and elastic. Erythrocyte Sp is a heterodimer composed of a 280 kDa alpha subunit and a 246 kDa beta subunit which associate in a side-to-side, antiparallel configuration to form a 100 nm rod-like structure. Sp in other tissues may be composed of distinct but homologous alpha and beta subunits, sometimes referred to as fodrin. A newly introduced nomenclature designates the Sp subunits of the erythrocyte as alpha-1 and beta-1, and the fodrin subunits as alpha-2 and beta-2. Alternatively spliced forms of each are designated as epsilon-1, epsilon-2, etc. (e. g. beta-1 epsilon-1).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: beta fodrin; beta II spectrin-like (short form); beta-fodrin; beta-G spectrin; Beta-II spectrin; beta-spectrin 2; beta-spectrin 2, non-erythrocytic; beta-spectrin II; beta-spectrin non-erythrocytic 1; brain spectrin; bSPII-; cytoskeleton protein; embryonic liver beta-fodrin; Embryonic liver fodrin; embryonic liver fodrin beta chain; epididymis luminal protein 102; Fodrin beta chain; non-erythrocytic; non-erythroid spectrin beta; short form of beta II spectrin; spectrin beta 2; spectrin beta chain, brain 1; Spectrin beta chain, non-erythrocytic 1; spectrin G; spectrin, beta, non-erythrocytic 1; Spectrin, non-erythroid beta chain 1

Gene Aliases: 9930031C03Rik; AL033301; betaSpII; ELF; elf1; elf3; HEL102; mKIAA4049; Spnb-2; Spnb1; Spnb2; SPTB2; SPTBN1

UniProt ID: (Human) Q01082, (Mouse) Q62261

Entrez Gene ID: (Human) 6711, (Rat) 305614, (Mouse) 20742

Function(s)