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SQSTM1 Recombinant Rabbit Monoclonal Antibody (R3F5)
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FIGURE: 1 / 11
SQSTM1 Antibody (MA572233) in ICC/IF
Immunocytochemistry analysis of SQSTM1 in HeLa cells. Permeabilized with Trinton X-100 at room temperature for 20 min. Blocking buffer (normal goat serum) at 37°C for 20 min. Samples were incubated with SQSTM1 Recombinant Monoclonal (Product # MA572233 using a dilution at 1:100, for 90 minutes at 37°C. Followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI was used to stain the cell nuclei.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA572233
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
R3F5
Immunogen
A synthesized peptide derived from human Sequestosome 1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: autophagy receptor p62; EBI3-associated protein of 60 kDa; EBI3-associated protein p60; oxidative stress induced; oxidative stress induced like; p62 transcript variant 4; p62 transcript variant 5; phosphotyrosine independent ligand for the Lck SH2 domain p62; phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; PKC-zeta-interacting protein; protein kinase C-zeta-interacting protein; ubiquitin-binding protein p62; unnamed protein product
Gene Aliases: A170; DMRV; EBIAP; FTDALS3; NADGP; OSF-6; Osi; OSIL; p60; p62; p62B; PDB3; STAP; STONE14; ZIP; ZIP3
UniProt ID: (Human) Q13501, (Mouse) Q64337, (Rat) O08623
Entrez Gene ID: (Human) 8878, (Mouse) 18412, (Rat) 113894
protein kinase C binding
protein binding
zinc ion binding
enzyme binding
protein kinase binding
protein binding, bridging
receptor tyrosine kinase binding
ubiquitin protein ligase binding
ionotropic glutamate receptor binding
signaling receptor activity
SH2 domain binding
identical protein binding
ubiquitin binding
macromolecular complex binding
K63-linked polyubiquitin binding
ubiquitin-dependent protein binding
protein sequestering activity
molecular sequestering activity
molecular condensate scaffold activity
transcription cofactor activity
signaling adaptor activity
negative regulation of transcription from RNA polymerase II promoter
mitophagy
pexophagy
temperature homeostasis
positive regulation of protein phosphorylation
response to ischemia
protein phosphorylation
ubiquitin-dependent protein catabolic process
protein import into nucleus
autophagy
endosome organization
positive regulation of autophagy
macroautophagy
protein catabolic process
negative regulation of protein ubiquitination
cellular response to stress
toll-like receptor 4 signaling pathway
negative regulation of toll-like receptor 4 signaling pathway
aggrephagy
regulation of I-kappaB kinase/NF-kappaB signaling
regulation of protein complex stability
brown fat cell proliferation
protein K63-linked ubiquitination
protein targeting to vacuole involved in autophagy
energy homeostasis
response to mitochondrial depolarisation
negative regulation of ferroptosis
membraneless organelle assembly
positive regulation of long-term synaptic potentiation
positive regulation of protein localization to plasma membrane
protein localization to perinuclear region of cytoplasm
mitophagy
protein localization
regulation of mitochondrion organization
endosomal transport
intracellular signal transduction
positive regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter
regulation of Ras protein signal transduction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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