Search
Agrisera
SRP43/Signal recognition peptide 43 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 1
SRP43/Signal recognition peptide 43 Antibody (AS12-2105) in WB
15 µg/well of total protein extracted freshly from rosetta leaves of Arabidopsis thaliana with 2 X LaemmLi buffer and denatured at 95°C/5 min. Samples were separated in the cold on 12% SDS-PAGE and blotted for 1.5 h to nitrocellulose (pore size of 0.45 µm), using: wet, semi-dry transfer in the cold. Blot was blocked with 5% milk for: 1 h/RT with agitation. Blot was incubated in the primary antibody at a dil... View More
Product Details
AS12-2105
Product Specifications
Species Reactivity
Plant
Host/Isotype
Chicken
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
SRP43 encodes a chloroplast-localized, soluble signal recognition particle subunit that accumulates in the stroma and functions at the thylakoid membrane during post-translational targeting. The protein is a plant-specific multidomain factor of ~43 kDa built from a central ankyrin-repeat region and an N-terminal chromodomain module (three chromodomains have been described in canonical cpSRP43 proteins), creating multiple linear-motif binding surfaces for partner recognition. Functionally, SRP43 is a core component of the chloroplast SRP pathway that forms a soluble transit complex with cpSRP54 and directly binds light-harvesting chlorophyll a/b-binding proteins (LHCPs) to prevent their aggregation after import into the chloroplast; the ankyrin repeats mediate high-affinity recognition of an LHCP motif and are sufficient for the ATP-independent chaperone activity, while chromodomain-dependent interactions contribute to docking and coordination with other pathway components such as the Alb3 insertase and cpSRP54 to promote efficient delivery and insertion of LHCPs into the thylakoid membrane.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: At2G47450; CAO; CPSRP43; T30B22.25
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support