Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 9
STAG1 Antibody (PA5-57115) in ICC/IF
Immunofluorescence analysis of Cohesin subunit SA-1 was performed using 70% confluent log phase MCF 10A cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with STAG1 Polyclonal Antibody (Product # PA5-57115) at 0.5 ug/ml in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Produ... View More
Product Details
PA5-57115
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human STAG1. Recombinant protein control fragment (Product #RP-96325).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2647987
Product Specific Information
Immunogen sequence: TSELPVLQDS TNETTAHSDA GSELEETEVK GKRKRGRPGR PPSTNKKPRK SPGEKSR
Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.
Target Information
This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Cohesin subunit SA-1; DKFZp781D1416; nuclear protein stromal antigen 1; SCC3 homolog 1; Stromal antigen 1
Gene Aliases: AU045003; SA-1; SA1; SCC3; SCC3A; STAG1
UniProt ID: (Human) Q8WVM7, (Mouse) Q9D3E6
Entrez Gene ID: (Human) 10274, (Mouse) 20842, (Rat) 315958
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support