Proteintech

STAM Polyclonal Antibody

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STAM Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 12434-1-AP (STAM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

STAM Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis tissue slide using 12434-1-AP ( STAM antibody) at dilution of 1:200 (under 40x lens).

STAM Antibody in Western Blot (WB) — mouse brain tissue were subjected to SDS PAGE followed by western blot with 12434-1-AP (STAM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

STAM Antibody in Immunoprecipitation (IP) — IP result of anti-STAM (IP:12434-1-AP, 4ug; Detection:12434-1-AP 1:300) with PC-3 cells lysate 1440ug.

Product Details

12434-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 7 publications 7 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 5 publications 5 publications
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	STAM Fusion Protein Ag3112 (54-403 aa encoded by BC030586) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.4 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: VNHKDPHVA MQALTLLGAC VSNCGKIFHL EVCSRDFASE VSNVLNKGHP KVCEKLKALM VEWTDEFKND PQLSLISAMI KNLKEQGVTF PAIGSQAAEQ AKASPALVAK DPGTVANKKE EEDLAKAIEL SLKEQRQQST TLSTLYPSTS SLLTNHQHEG RKVRAIYDFE AAEDNELTFK AGEIITVLDD SDPNWWKGET HQGIGLFPSN FVTAYLTAEP EMIKTEKKTV QFSDDVQVET IEPEPEPAFI DEDKMDQLLQ MLQSTDPSDD QPDLPELLHL EAMCHQMGPL IDEKLEDIDR KHSELSELNV KVMEALSLYT KLMNEDPMYS MYAKLQNQPG SGPTIRKPSP S (54-403 aa encoded by BC030586)

Target Information

STAM (Signal transducing adaptor molecule) is an adaptor molecule involved in downstream signaling pathways mediated by growth factors and cytokines. STAM also plays an important role in endocytosis, the JAK-STAT signaling pathway, and membrane trafficking. In humans, the gene encoding STAM is located on chromosome 10.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TIM-1 Ubiquitination Mediates Dengue Virus Entry.

Cell reports

Dejarnac O,Hafirassou ML,Chazal M,Versapuech M,Gaillard J,Perera-Lecoin M,Umana-Diaz C,Bonnet-Madin L,Carnec X,Tinevez JY,Delaugerre C,Schwartz O,Roingeard P,Jouvenet N,Berlioz-Torrent C,Meertens L,Amara A

Tue May 08 00:00:00 EDT 2018

TIM-1 Ubiquitination Mediates Dengue Virus Entry.

Cell reports

Tue May 08 00:00:00 EDT 2018

β-Arrestin1 and Signal-transducing Adaptor Molecule 1 (STAM1) Cooperate to Promote Focal Adhesion Kinase Autophosphorylation and Chemotaxis via the Chemokine Receptor CXCR4.

The Journal of biological chemistry

Alekhina O,Marchese A

Fri Dec 09 00:00:00 EST 2016

The α-Arrestin ARRDC3 Regulates the Endosomal Residence Time and Intracellular Signaling of the β2-Adrenergic Receptor.

The Journal of biological chemistry

Tian X,Irannejad R,Bowman SL,Du Y,Puthenveedu MA,von Zastrow M,Benovic JL

Fri Jul 08 00:00:00 EDT 2016

The α-Arrestin ARRDC3 Regulates the Endosomal Residence Time and Intracellular Signaling of the β2-Adrenergic Receptor.

The Journal of biological chemistry

Tian X,Irannejad R,Bowman SL,Du Y,Puthenveedu MA,von Zastrow M,Benovic JL

Fri Jul 08 00:00:00 EDT 2016

Identification of STAM1 as a novel effector of ventral projection of spinal motor neurons.

Development (Cambridge, England)

Nam H,Lee S

Fri Jul 01 00:00:00 EDT 2016

The endosomal sorting complex required for transport pathway mediates chemokine receptor CXCR4-promoted lysosomal degradation of the mammalian target of rapamycin antagonist DEPTOR.

The Journal of biological chemistry

Verma R,Marchese A

Fri Mar 13 00:00:00 EDT 2015

The ubiquitin ligase deltex-3l regulates endosomal sorting of the G protein-coupled receptor CXCR4.

Molecular biology of the cell

Holleman J,Marchese A

Sun Jun 15 00:00:00 EDT 2014

The ubiquitin ligase deltex-3l regulates endosomal sorting of the G protein-coupled receptor CXCR4.

Molecular biology of the cell

Holleman J,Marchese A

Sun Jun 15 00:00:00 EDT 2014

Novel roles for the E3 ubiquitin ligase atrophin-interacting protein 4 and signal transduction adaptor molecule 1 in G protein-coupled receptor signaling.

The Journal of biological chemistry

Malik R,Soh UJ,Trejo J,Marchese A

Fri Mar 16 00:00:00 EDT 2012

Novel roles for the E3 ubiquitin ligase atrophin-interacting protein 4 and signal transduction adaptor molecule 1 in G protein-coupled receptor signaling.

The Journal of biological chemistry

Malik R,Soh UJ,Trejo J,Marchese A

Fri Mar 16 00:00:00 EDT 2012

Arrestin-2 interacts with the endosomal sorting complex required for transport machinery to modulate endosomal sorting of CXCR4.

Molecular biology of the cell

Malik R,Marchese A

Thu Jul 15 00:00:00 EDT 2010

Arrestin-2 interacts with the endosomal sorting complex required for transport machinery to modulate endosomal sorting of CXCR4.

Molecular biology of the cell

Malik R,Marchese A

Thu Jul 15 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: DKFZp686J2352; HSE1 homolog; Signal transducing adapter molecule 1; signal transducing adaptor molecule (SH3 domain and ITAM motif) 1; STAM-1; STAT91

Gene Aliases: RGD1564499; STAM; STAM-1; STAM1

UniProt ID: (Human) Q92783, (Mouse) P70297

Entrez Gene ID: (Human) 8027, (Mouse) 20844, (Rat) 498798

Molecular Function: scaffold/adaptor protein