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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • STAM Antibodies

          Invitrogen

          STAM Polyclonal Antibody

          View all (16) STAM antibodies

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          Cite STAM Polyclonal Antibody

          • Antibody Testing Data (5)
          STAM Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          STAM Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          STAM Antibody (PA5-144659) in ICC/IF

          Immunocytochemistry analysis of STAM in SH-SY5Y cells. Cells were fixed in 4% paraformaldehyde for 30 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at 37&(8)451;. Cells were incubated with STAM polyclonal antibody (Product # PA5-144659) with a dilution of 1:200 in 2% BSA overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Nuclear DNA was labelled in blue with DAPI, beta tubulin (red) wa... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          STAM Antibody in Immunocytochemistry (ICC/IF)
          STAM Antibody in Immunocytochemistry (ICC/IF)
          STAM Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          STAM Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          STAM Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          STAM Polyclonal Antibody

          Product Details

          PA5-144659

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:200-1:600
          -

          Immunocytochemistry (ICC/IF)

          1:200
          -
          Product Specifications

          Species Reactivity

          Human, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide within Human aa 30-55/540.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 0.1% BSA, 40% glycerol

          Contains

          0.05% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_3091363

          Product Specific Information

          Positive control: Siha, SHSY5Y, human kidney tissue, human colon tissue, Rat testis tissue.

          Predicted band size: 59 kDa

          Subcellular Location: Cytoplasm; Early endosome membrane.

          Target Information

          STAM (Signal transducing adaptor molecule) is an adaptor molecule involved in downstream signaling pathways mediated by growth factors and cytokines. STAM also plays an important role in endocytosis, the JAK-STAT signaling pathway, and membrane trafficking. In humans, the gene encoding STAM is located on chromosome 10.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: DKFZp686J2352; HSE1 homolog; Signal transducing adapter molecule 1; signal transducing adaptor molecule (SH3 domain and ITAM motif) 1; STAM-1; STAT91

          View more View less

          Gene Aliases: RGD1564499; STAM; STAM-1; STAM1

          View more View less

          UniProt ID: (Human) Q92783

          View more View less

          Entrez Gene ID: (Human) 8027, (Rat) 498798

          View more View less

          Function(s)
          SH3/SH2 adaptor activity protein binding scaffold/adaptor protein
          Process(es)
          intracellular protein transport autophagy signal transduction positive regulation of signal transduction endosomal transport multivesicular body assembly negative regulation of epidermal growth factor receptor signaling pathway positive regulation of exosomal secretion regulation of extracellular exosome assembly
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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