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Product Details
12097-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Sheep
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
STK3 Fusion Protein Ag2741 (1-306 aa encoded by BC010640)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.14 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Product Specific Information
Immunogen sequence: MEQPPAPKS KLKKLSEDSL TKQPEEVFDV LEKLGEGSYG SVFKAIHKES GQVVAIKQVP VESDLQEIIK EISIMQQCDS PYVVKYYGSY FKNTDLWIVM EYCGAGSVSD IIRLRNKTLI EDEIATILKS TLKGLEYLHF MRKIHRDIKA GNILLNTEGH AKLADFGVAG QLTDTMAKRN TVIGTPFWMA PEVIQEIGYN CVADIWSLGI TSIEMAEGKP PYADIHPMRA IFMIPTNPPP TFRKPELWSD DFTDFVKKCL VKNPEQRATA TQLLQHPFIK NAKPVSILRD LITEAMEIKA KRHEEQQ (1-306 aa encoded by BC010640)
Target Information
STK3 (MST2) is a STE kinase family member that acts upstream of the MAP kinase signaling pathways, and is known to be activated by the proapoptotic stimuli staurosporine and FAS ligand. STK3 is a stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. It is a key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: KB-1458E12.1; Mammalian STE20-like protein kinase 2; MST-2; MST2/C; MST2/N; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog); Serine/threonine-protein kinase 3; Serine/threonine-protein kinase 3 20kDa subunit; Serine/threonine-protein kinase 3 36kDa subunit; Serine/threonine-protein kinase Krs-1; STE20-like kinase MST2
Gene Aliases: KRS1; MST2; STK3
UniProt ID: (Human) Q13188
Entrez Gene ID: (Human) 6788
Molecular Function:
non-receptor serine/threonine protein kinase
neural tube formation
endocardium development
protein phosphorylation
apoptotic process
signal transduction
central nervous system development
negative regulation of cell proliferation
signal transduction by protein phosphorylation
positive regulation of protein binding
hippo signaling
intracellular signal transduction
positive regulation of apoptotic process
positive regulation of fat cell differentiation
positive regulation of JNK cascade
negative regulation of organ growth
protein stabilization
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of protein kinase B signaling
primitive hemopoiesis
cell differentiation involved in embryonic placenta development
regulation of cell differentiation involved in embryonic placenta development
positive regulation of protein serine/threonine kinase activity
negative regulation of canonical Wnt signaling pathway
hepatocyte apoptotic process
positive regulation of extrinsic apoptotic signaling pathway via death domain receptors
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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