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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2,000 | View 1 publication 1 publication |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:20-1:200 | - |
Immunocytochemistry (ICC/IF) |
1:50-1:500 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Published species |
Sheep |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
STK3 Fusion Protein Ag2741 (1-306 aa encoded by BC010640) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.14 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.1% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: MEQPPAPKS KLKKLSEDSL TKQPEEVFDV LEKLGEGSYG SVFKAIHKES GQVVAIKQVP VESDLQEIIK EISIMQQCDS PYVVKYYGSY FKNTDLWIVM EYCGAGSVSD IIRLRNKTLI EDEIATILKS TLKGLEYLHF MRKIHRDIKA GNILLNTEGH AKLADFGVAG QLTDTMAKRN TVIGTPFWMA PEVIQEIGYN CVADIWSLGI TSIEMAEGKP PYADIHPMRA IFMIPTNPPP TFRKPELWSD DFTDFVKKCL VKNPEQRATA TQLLQHPFIK NAKPVSILRD LITEAMEIKA KRHEEQQ (1-306 aa encoded by BC010640)
STK3 is a stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. It is a key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: KB-1458E12.1; Mammalian STE20-like protein kinase 2; MST-2; MST2/C; MST2/N; serine/threonine kinase 3 (STE20 homolog, yeast); serine/threonine kinase 3 (Ste20, yeast homolog); Serine/threonine-protein kinase 3; Serine/threonine-protein kinase 3 20kDa subunit; Serine/threonine-protein kinase 3 36kDa subunit; Serine/threonine-protein kinase Krs-1; STE20-like kinase MST2
Gene Aliases: KRS1; MST2; STK3
UniProt ID: (Human) Q13188
Entrez Gene ID: (Human) 6788
Molecular Function:
non-receptor serine/threonine protein kinase
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