Invitrogen

STRA6 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Published Figure

3 References

View all (16) STRA6 antibodies

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Datasheet

Protocols

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STRA6 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of STRA6 in human cell line A549 using a STRA6 Polyclonal Antibody (Product # PA5-59319) shows localization to nucleus and cytosol.

STRA6 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of STRA6 in human testis using STRA6 Polyclonal Antibody (Product # PA5-59319) shows weak membranous positivity in cells in seminiferous ducts.

STRA6 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Fig. 5 The expression of human and mouse STRA6 in vitro and in vivo. a HepG2 cells were treated with 1 muM retinol in DMSO for 1 day, and the expression levels of the human STRA6 mRNA and protein were determined. The expression level of GAPDH was detected as an endogenous control. The experiments were performed in triplicate. The quantification of western blot data was performed using Image J and unpaired Student's t-tests were performed in Prism8. * P < 0.05. b The distributions of STRA6 and alphaSMA in normal (n = 3) and TAA-induced fibrotic mouse liver tissues (n = 3). STRA6 is shown in green, and alphaSMA is shown in red. The nuclei were stained with DAPI (blue). Scale bar: 25 mum. c The distributions of STRA6 and alphaSMA in normal (n = 3) and cirrhotic human liver tissues (n = 4). STRA6 is shown in green, and alphaSMA is shown in red. The nuclei were stained with DAPI (blue). Four samples in the cirrhosis group were analyzed independently, and representative data are shown in each panel. Scale bar of lower magnification: 25 mum; scale bar of higher magnification: 5 mum. d The accumulation of triglyceride in HepG2 cells treated with or without 1 muM retinol in DMSO for 24 h following transfection with a siRNA targeting human STRA6. Retinol autofluorescence is shown in blue, and triglyceride was stained using BODIPY (red). Retinol or triglyceride-positive area % per 0.03 mm 2 were quantified five images using Image J program. Reproducible result from three independent exp

STRA6 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of STRA6 demonstrates antibody specificity. Immunohistochemical analysis of STRA6 using anti-STRA6 Polyclonal Antibody (Product # PA5-59319), shows significant staining of STRA6 in human placenta and shows minimal or weak staining in human skin tissues. The relative expression levels of STRA6 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-59319

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:500	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human STRA6 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2648035

Product Specific Information

Immunogen sequence: YYTYRNFLKI EVSQSHPAMT AFCSLLLQAQ SLLPRTMAAP QDSLRPGEED EGMQLLQTKD SMAKGARPGA SRGRARWGLA YTLLHNPTLQ VFRKTALLGA NGAQP

Highest antigen sequence identity to the following orthologs: Mouse - 70%, Rat - 70%.

Target Information

STRA6 is a membrane protein involved in the metabolism of retinol. The encoded protein acts as a receptor for retinol/retinol binding protein complexes. This protein removes the retinol from the complex and transports it across the cell membrane. Defects in this gene are a cause of syndromic microphthalmia type 9 (MCOPS9).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Retinol from hepatic stellate cells via STRA6 induces lipogenesis on hepatocytes during fibrosis.

Cell & bioscience

Hwang I,Lee EJ,Park H,Moon D,Kim HS

PA5-59319 was used in Immunohistochemistry, Western Blot, Immunocytochemistry to demonstrate the role of STRA6-mediated retinol transfer from HSCs to hepatocytes in liver fibrosis.

Wed Jan 06 00:00:00 EST 2021

Retinol from hepatic stellate cells via STRA6 induces lipogenesis on hepatocytes during fibrosis.

Cell & bioscience

Hwang I,Lee EJ,Park H,Moon D,Kim HS

PA5-59319 was used in Immunohistochemistry, Western Blot, Immunocytochemistry to demonstrate the role of STRA6-mediated retinol transfer from HSCs to hepatocytes in liver fibrosis.

Wed Jan 06 00:00:00 EST 2021

Retinol from hepatic stellate cells via STRA6 induces lipogenesis on hepatocytes during fibrosis.

Cell & bioscience

Hwang I,Lee EJ,Park H,Moon D,Kim HS

PA5-59319 was used in Immunohistochemistry, Western Blot, Immunocytochemistry to demonstrate the role of STRA6-mediated retinol transfer from HSCs to hepatocytes in liver fibrosis.

Wed Jan 06 00:00:00 EST 2021

Retinol from hepatic stellate cells via STRA6 induces lipogenesis on hepatocytes during fibrosis.

Cell & bioscience

Hwang I,Lee EJ,Park H,Moon D,Kim HS

PA5-59319 was used in Immunohistochemistry, Western Blot, Immunocytochemistry to demonstrate the role of STRA6-mediated retinol transfer from HSCs to hepatocytes in liver fibrosis.

Wed Jan 06 00:00:00 EST 2021

Bioinformatics

Protein Aliases: FLJ12541; Receptor for retinol uptake STRA6; retinol binding protein 4 receptor; Retinol-binding protein receptor STRA6; stimulated by retinoic acid 6 homolog; stimulated by retinoic acid gene 6 homolog; Stimulated by retinoic acid gene 6 protein homolog

Gene Aliases: MCOPCB8; MCOPS9; PP14296; STRA6; UNQ3126/PRO10282/PRO19578

UniProt ID: (Human) Q9BX79

Entrez Gene ID: (Human) 64220

Function(s)

retinoid metabolic process blood vessel development kidney development pulmonary valve morphogenesis ventricular septum development heart development learning feeding behavior lung development adrenal gland development female genitalia development vocal learning ear development nose morphogenesis lung alveolus development positive regulation of behavior digestive tract morphogenesis embryonic digestive tract development developmental growth smooth muscle tissue development artery morphogenesis cognition neuromuscular process vitamin transport head development head morphogenesis face morphogenesis lung vasculature development diaphragm development embryonic camera-type eye formation eyelid development in camera-type eye uterus morphogenesis alveolar primary septum development pulmonary artery morphogenesis paramesonephric duct development ductus arteriosus closure

Process(es)

receptor activity vitamin transporter activity

STRA6 Polyclonal Antibody

Product Details

PA5-59319

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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