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Immunofluorescence analysis of mesenchymal stem cells using Zymed Ms anti-Stro-1 (Cat. No. 39-8401) and Gt anti-Mouse-Alexa Fluor 488 (Cat. No. A21042) (green). Tubulin is stained with phalloidin-Alexa 594 (red) and nuclei are stained with DAPI (blue). Sample is mounted in ProLong Gold antifade reagent.
|Tested species reactivity||Human|
|Published species reactivity||Rabbit, Human|
|Host / Isotype||Mouse / IgM|
|Immunogen||CD34+ human bone marrow cells|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow. STRO-1 binds to approximately 10% of bone marrow mononuclear cells, greater than 95% of which are nucleated erythroid precursors, but does not react with committed progenitor cells (colony-forming unit granulocyte-macrophage (CFU-GM), erythroid bursts (BFU-E), and mixed colonies (CFU-Mix). From bone marrow cells, the frequency of fibroblast colonyforming cells (CFU-F) is enriched approximately 100-fold in the STRO-1-positive/Glycophorin A-negative population than in the STRO-1-positive/Glycophorin A-positive population. When plated under long-term BM culture conditions, STRO-1-positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, and fibroblastic elements. A STRO-1-positive enriched subset of marrow cells is capable of differentiating into multiple mesenchymal lineages including hematopoiesis-supportive stromal cells with a vascular smooth muscle-like phenotype, adipocytes, osteoblasts and chondrocytes. STRO-1 is a valuable antibody for the identification, isolation and functional characterization of human bone marrow stromal cell precursors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mesenchymal stem cells in rabbit meniscus and bone marrow exhibit a similar feature but a heterogeneous multi-differentiation potential: superiority of meniscus as a cell source for meniscus repair.
39-8401 was used in immunocytochemistry to test if mesenchymal stem cells that reside in meniscus maintain specific traits are better for meniscus regeneration
|Ding Z,Huang H||BMC musculoskeletal disorders (16:null)||2015|
Differential properties of human ACL and MCL stem cells may be responsible for their differential healing capacity.
39-8401 was used in immunocytochemistry to characterize stem cells in human anterior cruciate and medial collateral ligaments.
|Zhang J,Pan T,Im HJ,Fu FH,Wang JH||BMC medicine (9:null)||2011|
|Human||Not Cited||Comparison of stem cell properties of cells isolated from normal and inflamed dental pulps.||Pereira LO,Rubini MR,Silva JR,Oliveira DM,Silva IC,Poças-Fonseca MJ,Azevedo RB||International endodontic journal (45:1080)||2012|
|Human||Not Cited||Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1.||Simmons PJ,Torok-Storb B||Blood (78:55)||1991|