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Invitrogen
SUMO1 Polyclonal Antibody
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FIGURE: 1 / 1
SUMO1 Antibody (PA5-96065) in WB
Frontiers in immunology 2023 -
Endotoxemia induced a distinct temporal and substrate specificity for splenic SUMO isoforms. Western-blots (top) and densitometric analyses (bottom) of splenic SUMOylation at the indicated post-LPS time points. Bacterial endotoxin induced SUMOylation-1 of (A) p150, (B) p100, (C) p250, and (D) SUMOylation-2/3 of p70 and p260 (smear). Comparative densitometric analyses of (E) SUMO1 and (F) SUMO2/3 at the indi... View More
Product Details
PA5-96065
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human SUMO1 (NP_0033431)
Conjugate
Form
Liquid
Concentration
2.45 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MSDQEAKPST EDLGDKKEGE YIKLKVIGQD SSEIHFKVKM TTHLKKLKES YCQRQGVPMN SLRFLFEGQR IADNHTPKEL GMEEEDVIEV YQEQTGGHST V; Positive Samples: HeLa, A-431, NCI-H460, SW480, Mouse brain, Mouse liver, Mouse testis, Rat brain, Rat testis; Cellular Location: Cell membrane, Cytoplasm, Nucleus, Nucleus membrane, Nucleus speckle, PML body
Target Information
SUMO1 is an ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment, via an isopeptide bond, to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. SUMO1 is involved, for instance, in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. SUMO1 may also regulate a network of genes involved in palate development. Mutations in the gene can result in non-syndromic orofacial cleft 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CG4494-PA; GAP modifying protein 1; GAP-modifying protein 1; GMP1; LD07775p; OTTHUMP00000202403; OTTHUMP00000202404; OTTHUMP00000202405; OTTHUMP00000202406; OTTHUMP00000202409; OTTHUMP00000202410; Sentrin; Sentrin-1; Small ubiquitin-related modifier 1; small ubiquitin-related modifier-1; SMT3 homolog 3; SMT3 suppressor of mif two 3 homolog 1; Smt3C; SUMO-1; SUMO-1 related peptidase; Ubiquitin-homology domain protein PIC1; ubiquitin-like 1; ubiquitin-like 1 (sentrin); Ubiquitin-like protein SMT3C; Ubiquitin-like protein UBL1
Gene Aliases: DAP1; GMP1; OFC10; OK/SW-cl.43; PIC1; SENP2; SENTRIN; SMT3; SMT3C; SMT3H3; SMTP3; SUMO-1; SUMO1; UBL1
UniProt ID: (Human) P63165, (Mouse) P63166, (Rat) Q5I0H3
Entrez Gene ID: (Human) 7341, (Mouse) 22218, (Rat) 301442
DNA repair
double-strand break repair via nonhomologous end joining
viral process
protein sumoylation
PML body organization
positive regulation of protein complex assembly
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
regulation of protein localization
negative regulation of DNA binding
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of action potential
negative regulation of transcription, DNA-templated
protein stabilization
palate development
regulation of interferon-gamma-mediated signaling pathway
global genome nucleotide-excision repair
regulation of cardiac muscle cell contraction
protein localization to nuclear pore
positive regulation of calcium-transporting ATPase activity
negative regulation of delayed rectifier potassium channel activity
regulation of transcription, DNA-templated
regulation of protein stability
regulation of calcium ion transmembrane transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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