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SURVIVIN Polyclonal Antibody

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SURVIVIN Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10508-1-AP (SURVIVIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SURVIVIN Antibody in Flow Cytometry (Flow) — 1X10^6 Jurkat cells were intracellularly stained with 0.2 µg Anti-Human SURVIVIN (Product # 10508-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.2 µg Control Antibody. Cells were fixed with 90% MeOH .

SURVIVIN Antibody in Immunoprecipitation (IP) — IP result of anti-SURVIVIN (IP: 10508-1-AP, 4 µg; Detection: 10508-1-AP 1:1000) with U2OS cells lysate 1000 µg.

Product Details

10508-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 37 publications 37 publications
Flow Cytometry (Flow)	Assay-dependant	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Hamster, Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SURVIVIN Fusion Protein Ag0788 (1-142 aa encoded by BC008718) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.41 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MGAPTLPPA WQPFLKDHRI STFKNWPFLE GCACTPERMA EAGFIHCPTE NEPDLAQCFF CFKELEGWEP DDDPIEEHKK HSSGCAFLSV KKQFEELTLG EFLKLDRERA KNKIAKETNN KKKEFEETAK KVRRAIEQLA AMD (1-142 aa encoded by BC008718)

Target Information

Survivin (IAP4) is the smallest member of the Inhibitors of Apoptosis Protein (IAP) gene family. The IAPs are involved in multiple cell functions such as cell signaling, cell division, metabolism, and exhibits differential expression in nearly all human cancers, but not in most normal tissues. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but the Survivin gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of survivin's gene expression. Survivin is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle in a specific and saturable reaction that is regulated by microtubule dynamics. Disruption of survivin-microtubule interactions results in loss of survivin's anti-apoptosis function and increased caspase-3 activity, a mechanism involved in cell death, during mitosis. Survivin may counteract a default induction of apoptosis in G2/M phase. Survivin is also abundantly expressed in brain tissues (astrocytes and some neurons) of adult rats following traumatic brain injury. Survivin has been found co-expressed with NeuN (mature neuronal marker) and PCNA (a cell cycle protein). Survivin might affect regulation of neural cell proliferative responses after brain injury. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. The overexpression of survivin in cancer may overcome this apoptotic checkpoint and favor aberrant progression of transformed cells through mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Apoptosis inhibitor 4; Apoptosis inhibitor survivin; Baculoviral IAP repeat-containing protein 5; BIRC 5; EPR 1; survivin; survivin variant 3 alpha; SVV; TIAP

Gene Aliases: AAC-11; AP14; API4; BIRC5; EPR-1; IAP4; survivin40; TIAP

UniProt ID: (Human) O15392, (Rat) Q9JHY7, (Mouse) O70201

Entrez Gene ID: (Human) 332, (Rat) 64041, (Mouse) 11799

Function(s)

G2/M transition of mitotic cell cycle microtubule cytoskeleton organization transcription, DNA-templated protein phosphorylation chromosome segregation regulation of mitotic cell cycle regulation of signal transduction protein ubiquitination protein complex localization positive regulation of exit from mitosis cellular response to stem cell factor stimulus response to drug negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process response to peptide hormone negative regulation of neuron apoptotic process positive regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of mitotic cell cycle cell division establishment of chromosome localization response to glucocorticoid regulation of insulin secretion involved in cellular response to glucose stimulus regulation of type B pancreatic cell proliferation cellular response to ethanol mitotic spindle assembly inhibition of cysteine-type endopeptidase activity involved in apoptotic process apoptotic process sister chromatid cohesion positive regulation of cell proliferation protein sumoylation regulation of apoptotic process cytokinesis regulation of transcription, DNA-templated cell cycle mitotic nuclear division embryo development negative regulation of peptidase activity spindle checkpoint

Process(es)

ubiquitin-protein transferase activity cysteine-type endopeptidase inhibitor activity protein binding microtubule binding Ran GTPase binding enzyme binding identical protein binding metal ion binding chaperone binding molecular_function zinc ion binding tubulin binding protein homodimerization activity cysteine-type endopeptidase inhibitor activity involved in apoptotic process protein heterodimerization activity cofactor binding peptidase inhibitor activity

SURVIVIN Polyclonal Antibody

Product Details

10508-1-AP

Western Blot (WB)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Immunohistochemistry (IHC)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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