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Immunohistochemistry analysis of formalin-fixed paraffin-embedded human tonsil germinal center stained using a SWAP70 polyclonal antibody (Product # PA5-23248) at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (14 HAFTALDQDHSGKVSKSQ 31) of SWAP70 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Western Blot (WB)||0.25-0.50 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
SWAP70 is a 585 amino acid regulatory protein belonging to the pleckstrin-like family with a PH domain essential for phosphatidylinositol 3, 4, 5-trisphosphate binding. SWAP70 acts as a link between the recognition of specific switch regions and causation of a DNA break and is involved in both nuclear events and in signaling B-cell activation. As a guanine nucleotide exchange factor (GEF) it transduces signals from tyrosine kinase receptors to RAC, independent of RAS. As a multiple functional signaling protein, and a component of SWAP enzyme complex, it mediates signaling of membrane ruffling by tyrosine kinase growth factor receptors, actin cytoskeleton and cell protrusion. It is widely expressed in most tissues, mature B-cells, including those associated with mucosa-associated tissue and bronchus-associated tissue, monocytes and macrophages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
HSPC321; switch-associated protein 70
HSPC321; KIAA0640; SWAP-70; SWAP70