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          • Primary Antibodies ›
          • SMAD4 Antibodies

          Fabgennix

          Smad4 Polyclonal Antibody

          View all (68) SMAD4 antibodies

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          Cite Smad4 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          Smad4 Antibody in Immunohistochemistry (IHC)
          Group 53 Created with Sketch.
          Smad4 Antibody in Immunohistochemistry (IHC)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Smad4 Antibody (SMAD4-421AP) in IHC

          Mouse Small Intestine- Smad4 Primary Antibody: SMAD4-421AP; 1:50 dilution in IHC Blocking Buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification. FFPE section. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Smad4 Antibody in Immunohistochemistry (IHC)
          Smad4 Polyclonal Antibody

          Product Details

          SMAD4-421AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          Immunohistochemistry (IHC)

          1:50-1:250
          -

          ELISA (ELISA)

          1:10,000
          -

          Immunoprecipitation (IP)

          1:50-1:250
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide taken within amino acid region 502-552 on human SMAD4 protein.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.5-1.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          proprietary buffer, pH 7.4-7.8, with 30% glycerol, 0.5% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Target Information

          Smad4 is encoded by the Smad4 gene that is located on chromosome 18 in humans. Smad4 is also known as Smad family member 4, Sma- and Mad-related protein 4 or Mothers against decapentaplegic (MAD) homolog 4. Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) globular domains that are involved in DNA binding and protein interactions respectively. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor -beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: Deleted in Pancreatic Carcinoma 4 (DPC4); deleted in pancreatic carcinoma locus 4; Deletion target in pancreatic carcinoma 4; Deletion target in pancreatic carcinoma 4 homolog; hSMAD4; MAD (mothers against decapentaplegic Drosophila) homolog 4; MAD homolog 4; MAD homolog 4 (MADH4); Mothers against decapentaplegic homolog 4; Mothers against decapentaplegic homolog 4 (MADH4); mothers against decapentaplegic, Drosophila, homolog of, 4; mothers against DPP homolog 4; SMAD; SMAD 4; SMAD family member 4; SMAD, mothers against DPP homolog 4; unnamed protein product

          View more View less

          Gene Aliases: AW743858; D18Wsu70e; DPC4; JIP; MADH4; MYHRS; SMAD4

          View more View less

          UniProt ID: (Human) Q13485, (Mouse) P97471, (Rat) O70437

          View more View less

          Entrez Gene ID: (Human) 4089, (Mouse) 17128, (Rat) 50554

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription corepressor binding transcription coactivator binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding collagen binding filamin binding identical protein binding protein homodimerization activity sulfate binding sequence-specific DNA binding macromolecular complex binding SMAD binding metal ion binding RNA polymerase II sequence-specific DNA binding transcription factor binding I-SMAD binding R-SMAD binding core promoter proximal region sequence-specific DNA binding transcription factor activity, protein binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding transforming growth factor beta receptor, common-partner cytoplasmic mediator activity transcription regulatory region DNA binding protein heterodimerization activity DNA-binding transcription factor gene-specific transcriptional regulator
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter ovarian follicle development osteoblast differentiation branching involved in ureteric bud morphogenesis response to hypoxia in utero embryonic development gastrulation with mouth forming second kidney development epithelial to mesenchymal transition outflow tract septum morphogenesis cardiac conduction system development atrioventricular valve formation epithelial to mesenchymal transition involved in endocardial cushion formation left ventricular cardiac muscle tissue morphogenesis positive regulation of cell proliferation involved in heart valve morphogenesis cardiac septum development brainstem development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter cellular iron ion homeostasis transforming growth factor beta receptor signaling pathway spermatogenesis single fertilization gastrulation axon guidance endoderm development mesoderm development cell proliferation negative regulation of cell proliferation male gonad development female gonad development anatomical structure morphogenesis anterior/posterior pattern specification regulation of gene expression negative regulation of cardiac muscle hypertrophy positive regulation of gene expression epithelial cell migration positive regulation of cardiac muscle cell apoptotic process positive regulation of epithelial to mesenchymal transition neural crest cell differentiation cardiac muscle hypertrophy in response to stress cell differentiation negative regulation of cell growth adrenal gland development BMP signaling pathway positive regulation of transforming growth factor beta receptor signaling pathway somite rostral/caudal axis specification regulation of transforming growth factor beta2 production activin receptor signaling pathway positive regulation of luteinizing hormone secretion intracellular signal transduction atrioventricular canal development endothelial cell activation regulation of cell proliferation negative regulation of protein catabolic process embryonic digit morphogenesis negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of follicle-stimulating hormone secretion mesendoderm development developmental growth neuron fate commitment neuron fate specification tissue morphogenesis sebaceous gland development formation of anatomical boundary regulation of hair follicle development uterus development positive regulation of SMAD protein import into nucleus SMAD protein signal transduction ventricular septum morphogenesis endocardial cell differentiation female gonad morphogenesis secondary palate development interleukin-6-mediated signaling pathway ERK1 and ERK2 cascade negative regulation of ERK1 and ERK2 cascade cellular response to glucose stimulus cellular response to growth factor stimulus response to transforming growth factor beta cellular response to transforming growth factor beta stimulus cellular response to BMP stimulus metanephric mesenchyme morphogenesis nephrogenic mesenchyme morphogenesis seminiferous tubule development regulation of primary metabolic process negative regulation of canonical Wnt signaling pathway extrinsic apoptotic signaling pathway transforming growth factor beta receptor superfamily signaling pathway positive regulation of extracellular matrix assembly positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of cardiac myofibril assembly SMAD protein complex assembly positive regulation of pathway-restricted SMAD protein phosphorylation regulation of transforming growth factor beta receptor signaling pathway positive regulation of BMP signaling pathway regulation of binding palate development negative regulation of cell death
          It has to be done as per old AB suggested Products section.

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