|Tested species reactivity||Human|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The epitope recognized by this antibody maps to a C-terminal region between residue 2340 and 2390 of spectrin beta 3.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||10-20 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||10-20 µg/ml|
|Immunoprecipitation (IP)||2-5 µg/mg lysate|
|Western Blot (WB)||1:2000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: Hela whole cell extract.
Mutations in spectrins are a previously unknown cause of ataxia and neurodegenerative disease that affect membrane proteins involved in glutamate signaling. Spectrin-beta IIIs have been recognized as ataxia disease genes and their mutations cause spinocerebellar ataxia type 5 (SCA5).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
A sensitive, reproducible and objective immunofluorescence analysis method of dystrophin in individual fibers in samples from patients with duchenne muscular dystrophy.
PA1-46007 was used in immunohistochemistry - frozen section to develop an immunofluorescence and semi-automated image analysis method to assess the sarcolemmal dystrophin intensity per individual fiber in a muscle biopsy
|Beekman C,Sipkens JA,Testerink J,Giannakopoulos S,Kreuger D,van Deutekom JC,Campion GV,de Kimpe SJ,Lourbakos A||PloS one (9:null)||2014|
Prednisolone treatment does not interfere with 2'-O-methyl phosphorothioate antisense-mediated exon skipping in Duchenne muscular dystrophy.
PA1-46007 was used in immunohistochemistry to study the effect of prednisolone treatment on exon skipping in Duchenne muscular dystrophy
|Verhaart IE,Heemskerk H,Karnaoukh TG,Kolfschoten IG,Vroon A,van Ommen GJ,van Deutekom JC,Aartsma-Rus A||Human gene therapy (23:262)||2012|