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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full length recombinant human Ssu72 protein.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is MCF-7 or COS-7 cells.
Human Ssu72 is a highly conserved homologue of yeast Ssu72, a CTD phosphatase and a component of the polyadenylation/ termination machinery. The human Ssu72 is located on Chromosome 1p36 and seems to represent a single gene. It was identified as a pRb binding factor and was also shown to interact with TFIIB and yeast PTA1. Rabbit polyclonal antibodies were raised against SSU72 fusion protein. The ABs recognize a single band of ~30 Kda on immunoblots of various human cell lines but do not work well on mouse tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CTD phosphatase SSU72; FLJ13048; HSPC182; PNAS-120; RNA polymerase II subunit A C-terminal domain phosphatase SSU72; RP5-832C2.2
HSPC182; PNAS-120; SSU72