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Invitrogen
Supt4h1 Polyclonal Antibody
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FIGURE: 1 / 1
Supt4h1 Antibody (PA5-96867) in WB
Western blot analysis of Supt4h1 in extracts of various cell lines. Samples were incubated with Supt4h1 Polyclonal antibody (Product # PA5-96867) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Product Details
PA5-96867
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human SUPT4H1 (NP_0031591)
Conjugate
Form
Liquid
Concentration
1.82 mg/mL
Amount
182 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MALETVPKDL RHLRACLLCS LVKTIDQFEY DGCDNCDAYL QMKGNREMVY DCTSSSFDGI IAMMSPEDSW VSKWQRVSNF KPGVYAVSVT GRLPQGIVRE LKSRGVAYKS RDTAIKT; Positive Samples: MCF7, B-cell; Cellular Location: Nucleus
Target Information
Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DRB sensitivity-inducing factor 14 kDa subunit; DRB sensitivity-inducing factor small subunit; DSIF p14; DSIF small subunit; hSPT4; similar to Saccharomyces cerevisiae Spt4; protein has potential N-terminal zinc-finger; small hSpt4 subunit; suppressor of Ty 4 homolog 1; SUPT4H1; Transcription elongation factor SPT4; unnamed protein product
Gene Aliases: SPT4; SPT4H; Supt4a; SUPT4H; SUPT4H1
UniProt ID: (Human) P63272
Entrez Gene ID: (Human) 6827
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
transcription elongation from RNA polymerase II promoter
negative regulation of DNA-templated transcription, elongation
positive regulation of DNA-templated transcription, elongation
regulation of transcription elongation from RNA polymerase II promoter
negative regulation of transcription elongation from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
transcription elongation-coupled chromatin remodeling
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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