Invitrogen

Syntaxin 4 Polyclonal Antibody

View all (13) Syntaxin 4 antibodies

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Syntaxin 4 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence staining of Syntaxin 4 in U251MG cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Syntaxin 4 Polyclonal Antibody (Product # PA5-118290, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and cell membrane.

Syntaxin 4 Antibody in Western Blot (WB) — Western Blot using Syntaxin 4 Polyclonal Antibody (Product # PA5-118290) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: 293 Whole Cell Lysate, Lane C: K562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 36 kDa.

Syntaxin 4 Antibody in Immunoprecipitation (IP) — Syntaxin 4 Immunoprecipitation using: Lane A: 0.5 mg K562 Whole Cell Lysate 4 µL with Syntaxin 4 Polyclonal Antibody (Product # PA5-118290) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: Syntaxin 4 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 36 kDa.

Product Details

PA5-118290

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-
Immunoprecipitation (IP)	1-5 μL/mg of lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E. coli-derived Human STX4 fragment
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography, Protein A
Storage buffer	PBS, pH 7
Contains	0.03% Proclin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2902887

Target Information

STX4 is a plasma membrane t-SNARE that mediates docking of transport vesicles.STX4 is necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, STX4 may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes. STX4 may also play a role in docking of synaptic vesicles at presynaptic active zones.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Renal carcinoma antigen NY-REN-31; syntaxin 4A (placental); Syntaxin-4

Gene Aliases: p35-2; STX4; STX4A

UniProt ID: (Human) Q12846

Entrez Gene ID: (Human) 6810

Molecular Function: SNARE protein

intracellular protein transport post-Golgi vesicle-mediated transport positive regulation of cell proliferation regulation of exocytosis positive regulation of cell migration synaptic vesicle fusion to presynaptic active zone membrane response to hydroperoxide SNARE complex assembly positive regulation of insulin secretion involved in cellular response to glucose stimulus positive regulation of catalytic activity positive regulation of eosinophil degranulation positive regulation of cell adhesion vesicle docking organelle fusion positive regulation of chemotaxis positive regulation of immunoglobulin secretion long-term synaptic potentiation positive regulation of establishment of protein localization to plasma membrane regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of protein localization to plasma membrane positive regulation of protein localization to cell surface

Process(es)

SNARE binding SNAP receptor activity protein binding sphingomyelin phosphodiesterase activator activity myosin binding